Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA0519
|
Name |
nirS
|
Replicon | chromosome |
Genomic location | 578394 - 580100 (- strand) |
Transposon Mutants | 13 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
RefSeq | NP_249210.1 |
GI | 15595716 |
Affymetrix | PA0519_nirS_at |
Entrez | 882217 |
GenBank | AAG03908.1 |
INSDC | CAA35702.1 |
NCBI Locus Tag | PA0519 |
protein_id(GenBank) | gb|AAG03908.1|AE004488_12|gnl|PseudoCAP|PA0519 |
TIGR | NTL03PA00520 |
UniParc | UPI0000055F2D |
UniProtKB Acc | P24474 |
UniProtKB ID | NIRS_PSEAE |
UniRef100 | UniRef100_P24474 |
UniRef50 | UniRef50_P24474 |
UniRef90 | UniRef90_P24474 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
nitrite reductase precursor
|
Synonyms |
cytochrome oxidase cytochrome cd1 |
Evidence for Translation |
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | 5.04 |
Kyte-Doolittle Hydrophobicity Value | -0.391 |
Molecular Weight (kDa) | 62.7 |
Isoelectric Point (pI) | 8.21 |
Individual Mappings | |
Additional evidence for subcellular localization |
Detected in a periplasmic protein preparation by spheroplasting by lysozyme and sucrose.(PMID:19333994)
|
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
6TPO | OXIDOREDUCTASE | 12/13/19 | Conformation of cd1 nitrite reductase NirS without bound heme d1 | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 1.861 | X-RAY DIFFRACTION | 100.0 |
1N90 | OXIDOREDUCTASE | 09/04/98 | FOLLOWING THE C HEME REDUCTION IN NITRITE REDUCTASE FROM PSEUDOMONAS AERUGINOSA | Pseudomonas aeruginosa | 2.9 | X-RAY DIFFRACTION | 100.0 |
1NIR | NITRITE REDUCTASE | 06/17/97 | OXYDIZED NITRITE REDUCTASE FROM PSEUDOMONAS AERUGINOSA | Pseudomonas aeruginosa | 2.15 | X-RAY DIFFRACTION | 100.0 |
5GUW | MEMBRANE PROTEIN | 08/31/16 | Complex of Cytochrome cd1 Nitrite Reductase and Nitric Oxide Reductase in Denitrification of Pseudomonas aeruginosa | Pseudomonas aeruginosa PAO1 | 3.2 | X-RAY DIFFRACTION | 100.0 |
1N15 | OXIDOREDUCTASE | 09/04/98 | FOLLOWING THE C HEME REDUCTION IN NITRITE REDUCTASE FROM PSEUDOMONAS AERUGINOSA | Pseudomonas aeruginosa | 2.9 | X-RAY DIFFRACTION | 100.0 |
1HZU | OXIDOREDUCTASE | 01/26/01 | DOMAIN SWING UPON HIS TO ALA MUTATION IN NITRITE REDUCTASE OF PSEUDOMONAS AERUGINOSA | Pseudomonas aeruginosa | 2.7 | X-RAY DIFFRACTION | 99.8 |
1HZV | OXIDOREDUCTASE | 01/26/01 | DOMAIN SWING UPON HIS TO ALA MUTATION IN NITRITE REDUCTASE OF PSEUDOMONAS AERUGINOSA | Pseudomonas aeruginosa | 2.83 | X-RAY DIFFRACTION | 99.8 |
1BL9 | OXIDOREDUCTASE | 07/20/98 | CONFORMATIONAL CHANGES OCCURRING UPON REDUCTION IN NITRITE REDUCTASE FROM PSEUDOMONAS AERUGINOSA | Pseudomonas aeruginosa | 2.9 | X-RAY DIFFRACTION | 100.0 |
1NNO | OXIDOREDUCTASE | 07/20/98 | CONFORMATIONAL CHANGES OCCURRING UPON NO BINDING IN NITRITE REDUCTASE FROM PSEUDOMONAS AERUGINOSA | Pseudomonas aeruginosa | 2.65 | X-RAY DIFFRACTION | 100.0 |
6TSI | OXIDOREDUCTASE | 12/20/19 | cd1 nitrite reductase NirS with bound dihydro-heme d1 | Pseudomonas aeruginosa PAO1 | 2.38 | X-RAY DIFFRACTION | 100.0 |
1GJQ | OXIDOREDUCTASE | 08/01/01 | Pseudomonas aeruginosa cd1 nitrite reductase reduced cyanide complex | PSEUDOMONAS AERUGINOSA | 2.7 | X-RAY DIFFRACTION | 100.0 |
1N50 | OXIDOREDUCTASE | 09/04/98 | FOLLOWING THE C HEME REDUCTION IN NITRITE REDUCTASE FROM PSEUDOMONAS AERUGINOSA | Pseudomonas aeruginosa | 2.9 | X-RAY DIFFRACTION | 100.0 |
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
Heme C | DB03317 | DrugBank | 5.1.4 | P24474 | Nitrite reductase | 100.0 | 568 | 0.0e+00 |
Heme D | DB03469 | DrugBank | 5.1.4 | P24474 | Nitrite reductase | 100.0 | 568 | 0.0e+00 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 48 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000503 (354 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA0519
Search term: nirS
Search term: nitrite reductase precursor
|
Nitrite reductase from Pseudomonas aeruginosa: sequence of the gene and the protein.
Silvestrini MC, Galeotti CL, Gervais M, Schininà E, Barra D, Bossa F, Brunori M
FEBS Lett 1989 Aug 28;254(1-2):33-8
PubMed ID: 2506077
|
The structural gene for cytochrome c551 from Pseudomonas aeruginosa. The nucleotide sequence shows a location downstream of the nitrite reductase gene.
Nordling M, Young S, Karlsson BG, Lundberg LG
FEBS Lett 1990 Jan 1;259(2):230-2
PubMed ID: 2152881
|
N-terminal arm exchange is observed in the 2.15 A crystal structure of oxidized nitrite reductase from Pseudomonas aeruginosa.
Nurizzo D, Silvestrini MC, Mathieu M, Cutruzzolà F, Bourgeois D, Fülöp V, Hajdu J, Brunori M, Tegoni M, Cambillau C
Structure 1997 Sep 15;5(9):1157-71
PubMed ID: 9331415
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
NO production by Pseudomonas aeruginosa cd1 nitrite reductase.
Cutruzzolà F, Rinaldo S, Centola F, Brunori M
IUBMB Life 2003 Oct-Nov;55(10-11):617-21
PubMed ID: 14711008
|