Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA2000
|
Name |
dhcB
Synonym: dhcB Synonym: atoA Synonym: scoB |
Replicon | chromosome |
Genomic location | 2186260 - 2186916 (+ strand) |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
Comment | Deletion of dhcB or transposon insertions into the coding sequence result in a strain unable to grow using L-carnitine as a sole carbon source. DhcB is predicted to form a complex with DhcA which functions to deacetylate 3-dehydrocarnitine generating acetyl-CoA and glycine betaine CoA. Deletion of the dhcAB operon results in complete loss of carnitine-dependent CoA utilization from cytoplasmic extracts compared to cytoplasm from wild-type cells.;Transcription of the dhcAB operon is regulated by the transcription factor DhcR. |
RefSeq | NP_250690.1 |
GI | 15597196 |
Affymetrix | PA2000_at |
DNASU | PaCD00006895 |
Entrez | 878555 |
GenBank | AAG05388.1 |
INSDC | AAG05388.1 |
NCBI Locus Tag | PA2000 |
protein_id(GenBank) | gb|AAG05388.1|AE004626_7|gnl|PseudoCAP|PA2000 |
TIGR | NTL03PA02000 |
UniParc | UPI00000C554F |
UniProtKB Acc | Q9I2A9 |
UniProtKB ID | Q9I2A9_PSEAE |
UniRef100 | UniRef100_Q9I2A9 |
UniRef50 | UniRef50_Q9I2A9 |
UniRef90 | UniRef90_Q9I2A9 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
dehydrocarnitine CoA transferase, subunit B
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -8.37 |
Kyte-Doolittle Hydrophobicity Value | 0.243 |
Molecular Weight (kDa) | 23.2 |
Isoelectric Point (pI) | 4.57 |
Individual Mappings | |
Additional evidence for subcellular localization |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 271 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG003435 (528 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA2000
Search term: dhcB
Search term: dehydrocarnitine CoA transferase, subunit B
|
Ensembl
110, assembly
GRCh38.p14
|
3-oxoacid CoA-transferase 1 [Source:HGNC Symbol;Acc:HGNC:8527]
E-value:
8.0e-55
Percent Identity:
51.7
|
Ensembl
110, assembly
GRCh38.p14
|
3-oxoacid CoA-transferase 1 [Source:HGNC Symbol;Acc:HGNC:8527]
E-value:
8.0e-55
Percent Identity:
51.7
|
Identification of glutamate 344 as the catalytic residue in the active site of pig heart CoA transferase.
Rochet JC, Bridger WA
Protein Sci 1994 Jun;3(6):975-81
PubMed ID: 7915164
|
Molecular characterization of lysR-lysXE, gcdR-gcdHG and amaR-amaAB operons for lysine export and catabolism: a comprehensive lysine catabolic network in Pseudomonas aeruginosa PAO1.
Madhuri Indurthi S, Chou HT, Lu CD
Microbiology (Reading) 2016 May;162(5):876-888
PubMed ID: 26967762
|