Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA3361
|
Name |
lecB
|
Replicon | chromosome |
Genomic location | 3773029 - 3773376 (+ strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Comment | Basolaterally applied LecB binds beta1-integrin and causes its internalization (PMID:32156827) |
Comment | LecB inhibits cell migration and epithelial wound healing (PMID:32156827) |
RefSeq | NP_252051.1 |
GI | 15598557 |
Affymetrix | PA3361_at |
DNASU | PaCD00007472 |
Entrez | 882528 |
GenBank | AAG06749.1 |
INSDC | AAG06749.1 |
NCBI Locus Tag | PA3361 |
protein_id(GenBank) | gb|AAG06749.1|AE004758_6|gnl|PseudoCAP|PA3361 |
TIGR | NTL03PA03361 |
UniParc | UPI00000C59D3 |
UniProtKB Acc | Q9HYN5 |
UniProtKB ID | Q9HYN5_PSEAE |
UniRef100 | UniRef100_W0YU60 |
UniRef50 | UniRef50_Q02QJ5 |
UniRef90 | UniRef90_W0YU60 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
fucose-binding lectin PA-IIL
|
Synonyms |
LecB |
Evidence for Translation | |
Charge (pH 7) | -5.02 |
Kyte-Doolittle Hydrophobicity Value | 0.054 |
Molecular Weight (kDa) | 11.9 |
Isoelectric Point (pI) | 3.83 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
6S5R | SUGAR BINDING PROTEIN | 07/02/19 | Cfucosylated second generation peptide dendrimer SBD6 bound to Fucose binding Lectin LecB (PA-IIL) from Pseudomonas aeruginosa at 2.08 Angstrom resolution, incomplete structure | Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT | 2.076 | X-RAY DIFFRACTION | 100.0 |
2JDH | SUGAR BINDING PROTEIN | 01/09/07 | Lectin PA-IIL of P.aeruginosa complexed with disaccharide derivative | PSEUDOMONAS AERUGINOSA | 1.1 | X-RAY DIFFRACTION | 100.0 |
1GZT | LECTIN | 06/06/02 | Pseudomonas aeruginosa lectin II (PA-IIL) together with fucose | PSEUDOMONAS AERUGINOSA | 1.3 | X-RAY DIFFRACTION | 100.0 |
2JDK | LECTIN | 01/10/07 | Lectin PA-IIL of P.aeruginosa complexed with disaccharide derivative | PSEUDOMONAS AERUGINOSA | 1.1 | X-RAY DIFFRACTION | 100.0 |
6S5P | SUGAR BINDING PROTEIN | 07/02/19 | Cfucosylated peptide SBL2 bound to Fucose binding Lectin LecB (PA-IIL) from Pseudomonas aeruginosa at 1.46 Angstrom resolution | Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT | 1.46 | X-RAY DIFFRACTION | 100.0 |
7NEW | ANTIBIOTIC | 02/05/21 | Fucosylated heterochiral linear peptide Fdln69 bound to the fucose binding lectin LecB PA-IIL from Pseudomonas aeruginosa at 2.0 Angstrom resolution | Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT | 2.02 | X-RAY DIFFRACTION | 100.0 |
7NEF | ANTIBIOTIC | 02/03/21 | Fucosylated linear peptide Fln65 bound to the fucose binding lectin LecB PA-IIL from Pseudomonas aeruginosa at 1.5 Angstrom resolution | Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT | 1.51 | X-RAY DIFFRACTION | 100.0 |
3ZDV | SUGAR BINDING PROTEIN | 11/30/12 | Crystal structure of the LecB lectin from Pseudomonas aeruginosa in complex with Methyl 6-(2,4,6-trimethylphenylsulfonylamido)-6-deoxy-alpha-D-mannopyranoside | PSEUDOMONAS AERUGINOSA | 1.41 | X-RAY DIFFRACTION | 100.0 |
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
N-acetyl-alpha-D-glucosamine | DB03740 | DrugBank | 5.1.4 | Q9HYN5 | Fucose-binding lectin PA-IIL | 100.0 | 115 | 3.3e-55 |
Beta-D-Glucose | DB02379 | DrugBank | 5.1.4 | Q9HYN5 | Fucose-binding lectin PA-IIL | 100.0 | 115 | 3.3e-55 |
Beta-D-Fructopyranose | DB02561 | DrugBank | 5.1.4 | Q9HYN5 | Fucose-binding lectin PA-IIL | 100.0 | 115 | 3.3e-55 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 7 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG002267 (300 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA3361
Search term: lecB
Search term: fucose-binding lectin PA-IIL
|
Structural basis for oligosaccharide-mediated adhesion of Pseudomonas aeruginosa in the lungs of cystic fibrosis patients.
Mitchell E, Houles C, Sudakevitz D, Wimmerova M, Gautier C, Pérez S, Wu AM, Gilboa-Garber N, Imberty A
Nat Struct Biol 2002 Dec;9(12):918-21
PubMed ID: 12415289
|
Identification and characterization of pseudomonas aeruginosa PA-IIL lectin gene and protein compared to PA-IL.
Gilboa-Garber N, Katcoff DJ, Garber NC
FEMS Immunol Med Microbiol 2000 Sep;29(1):53-7
PubMed ID: 10967261
|
Pseudomonas aeruginosa lectin LecB is located in the outer membrane and is involved in biofilm formation.
Tielker D, Hacker S, Loris R, Strathmann M, Wingender J, Wilhelm S, Rosenau F, Jaeger KE
Microbiology (Reading) 2005 May;151(Pt 5):1313-1323
PubMed ID: 15870442
|
The Pseudomonas aeruginosa Lectin LecB Causes Integrin Internalization and Inhibits Epithelial Wound Healing.
Thuenauer R, Landi A, Trefzer A, Altmann S, Wehrum S, Eierhoff T, Diedrich B, Dengjel J, Nyström A, Imberty A, Römer W
mBio 2020 Mar 10;11(2)
PubMed ID: 32156827
|