Pseudomonas aeruginosa PAO1, PA4834 (cntI)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4834
Name
cntI
Synonym: zrmD
Replicon chromosome
Genomic location 5424761 - 5425615 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
Commentpart of the Zur-regulated metallophore operon known as cntOLMI (PMID:29214991) or zrmABCD(PMID:28898501).

Cross-References

RefSeq NP_253521.1
GI 15600027
Affymetrix PA4834_at
DNASU PaCD00008099
Entrez 882351
GenBank AAG08219.1
INSDC AAG08219.1
NCBI Locus Tag PA4834
protein_id(GenBank) gb|AAG08219.1|AE004896_9|gnl|PseudoCAP|PA4834
TIGR NTL03PA04835
UniParc UPI00000C5E2F
UniProtKB Acc Q9HUX6
UniProtKB ID Q9HUX6_PSEAE
UniRef100 UniRef100_Q9HUX6
UniRef50 UniRef50_Q9HUX6
UniRef90 UniRef90_Q9HUX6

Product

Feature Type CDS
Coding Frame 1
Product Name
CntI
Product Name Confidence: Class 3
Synonyms putative nicotianamine synthase
hypothetical protein
Evidence for Translation
Charge (pH 7) 7.12
Kyte-Doolittle Hydrophobicity Value 1.163
Molecular Weight (kDa) 30.7
Isoelectric Point (pI) 9.65

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 247 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004465 (299 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4834
Search term: cntI
Search term: CntI

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
A novel siderophore system is essential for the growth of Pseudomonas aeruginosa in airway mucus.
Gi M, Lee KM, Kim SC, Yoon JH, Yoon SS, Choi JY
Sci Rep 2015 Oct 8;5:14644
PubMed ID: 26446565
Growth of Pseudomonas aeruginosa in zinc poor environments is promoted by a nicotianamine-related metallophore.
Mastropasqua MC, D'Orazio M, Cerasi M, Pacello F, Gismondi A, Canini A, Canuti L, Consalvo A, Ciavardelli D, Chirullo B, Pasquali P, Battistoni A
Mol Microbiol 2017 Nov;106(4):543-561
PubMed ID: 28898501
Pseudomonas aeruginosa zinc uptake in chelating environment is primarily mediated by the metallophore pseudopaline.
Lhospice S, Gomez NO, Ouerdane L, Brutesco C, Ghssein G, Hajjar C, Liratni A, Wang S, Richaud P, Bleves S, Ball G, Borezée-Durant E, Lobinski R, Pignol D, Arnoux P, Voulhoux R
Sci Rep 2017 Dec 7;7(1):17132
PubMed ID: 29214991