Pseudomonas aeruginosa PAO1, PA4921 (choE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4921
Name
choE
Replicon chromosome
Genomic location 5520672 - 5521595 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs
CommentGene changed names between the authors' original submission and the paper acceptance.

Cross-References

RefSeq NP_253608.1
GI 15600114
Affymetrix PA4921_at
Entrez 878045
GenBank AAG08306.1
INSDC AHL26984.1
NCBI Locus Tag PA4921
protein_id(GenBank) gb|AAG08306.1|AE004905_4|gnl|PseudoCAP|PA4921
TIGR NTL03PA04922
UniParc UPI00000C5E7D
UniProtKB Acc Q9HUP2
UniProtKB ID Q9HUP2_PSEAE
UniRef100 UniRef100_Q9HUP2
UniRef50 UniRef50_Q9HUP2
UniRef90 UniRef90_Q9HUP2

Product

Feature Type CDS
Coding Frame 1
Product Name
cholinesterase, ChoE
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) 4.31
Kyte-Doolittle Hydrophobicity Value -0.227
Molecular Weight (kDa) 33.5
Isoelectric Point (pI) 8.46

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Extracellular Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6UQY HYDROLASE 10/21/19 Crystal structure of ChoE H288N mutant in complex with acetylthiocholine Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.57 X-RAY DIFFRACTION 99.7
6UQX HYDROLASE 10/21/19 Crystal structure of ChoE in complex with propionylthiocholine Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.85 X-RAY DIFFRACTION 100.0
6UQV HYDROLASE 10/21/19 Crystal structure of ChoE, a bacterial acetylcholinesterase from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.35 X-RAY DIFFRACTION 100.0
8D8W HYDROLASE 06/09/22 Crystal structure of ChoE with Ser38 adopting alternative conformations Pseudomonas aeruginosa 1.4 X-RAY DIFFRACTION 100.0
6UR0 HYDROLASE 10/21/19 Crystal structure of ChoE D285N mutant acyl-enzyme Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.8 X-RAY DIFFRACTION 99.3
8D8Y HYDROLASE 06/09/22 Crystal structure of ChoE N147A mutant in complex with acetylthiocholine Pseudomonas aeruginosa 1.54 X-RAY DIFFRACTION 99.7
6UQW HYDROLASE 10/21/19 Crystal structure of ChoE in complex with acetate and thiocholine Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.65 X-RAY DIFFRACTION 100.0
8D8Z HYDROLASE 06/09/22 Crystal structure of ChoE N147A mutant in complex with thiocholine and chloride Pseudomonas aeruginosa 1.38 X-RAY DIFFRACTION 99.7
8D90 HYDROLASE 06/09/22 Crystal structure of ChoE N147A mutant in complex with bromide ions Pseudomonas aeruginosa 1.82 X-RAY DIFFRACTION 99.7
6UQZ HYDROLASE 10/21/19 Crystal structure of ChoE D285N mutant in complex with acetate and thiocholine Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.43 X-RAY DIFFRACTION 99.7
6UR1 HYDROLASE 10/21/19 Crystal structure of ChoE S38A mutant in complex with acetate and acetylthiocholine Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.42 X-RAY DIFFRACTION 99.7
8D8X HYDROLASE 06/09/22 Crystal structure of ChoE in complex with acetate and thiocholine (crystal form 2) Pseudomonas aeruginosa 1.36 X-RAY DIFFRACTION 100.0
8D91 HYDROLASE 06/09/22 Crystal structure of ChoE in complex with acetate and tetraethylammonium (TEA) Pseudomonas aeruginosa 1.49 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 72 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004538 (814 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4921
Search term: choE

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Molecular cloning of a phospholipid-cholesterol acyltransferase from Aeromonas hydrophila. Sequence homologies with lecithin-cholesterol acyltransferase and other lipases.
Thornton J, Howard SP, Buckley JT
Biochim Biophys Acta 1988 Mar 25;959(2):153-9
PubMed ID: 3280033
A Pseudomonas aeruginosa PAO1 acetylcholinesterase is encoded by the PA4921 gene and belongs to the SGNH hydrolase family.
Sánchez DG, Otero LH, Hernández CM, Serra AL, Encarnación S, Domenech CE, Lisa AT
Microbiol Res 2012 Jun 20;167(6):317-25
PubMed ID: 22192836