Pseudomonas aeruginosa PAO1, PA5317 (dppA5)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5317
Name
dppA5
Replicon chromosome
Genomic location 5986475 - 5988055 (+ strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentStructural similarity to 1DPE (dipeptide binding protein from Escherichia coli, root-mean-square deviation: 0.6 angstroms). Sequence similarity to 1DPE (99%/44,86%) (G-L). Final predicted ligand: dipeptide (PMID: 31631799)

Cross-References

RefSeq NP_254004.1
GI 15600510
Affymetrix PA5317_at
Entrez 880892
GenBank AAG08702.1
INSDC AAG08702.1
NCBI Locus Tag PA5317
protein_id(GenBank) gb|AAG08702.1|AE004944_6|gnl|PseudoCAP|PA5317
TIGR NTL03PA05318
UniParc UPI00000C5FA2
UniProtKB Acc Q9HTN7
UniProtKB ID Q9HTN7_PSEAE
UniRef100 UniRef100_S0HDK8
UniRef50 UniRef50_W6R3H6
UniRef90 UniRef90_A6VEC4

Product

Feature Type CDS
Coding Frame 1
Product Name
probable binding protein component of ABC dipeptide transporter
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.22
Kyte-Doolittle Hydrophobicity Value -0.226
Molecular Weight (kDa) 57.9
Isoelectric Point (pI) 6.96

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 548 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004888 (506 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5317
Search term: dppA5

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Peptide transport and chemotaxis in Escherichia coli and Salmonella typhimurium: characterization of the dipeptide permease (Dpp) and the dipeptide-binding protein.
Abouhamad WN, Manson M, Gibson MM, Higgins CF
Mol Microbiol 1991 May;5(5):1035-47
PubMed ID: 1956284
Crystal structure of the dipeptide binding protein from Escherichia coli involved in active transport and chemotaxis.
Dunten P, Mowbray SL
Protein Sci 1995 Nov;4(11):2327-34
PubMed ID: 8563629
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
High-throughput screening of dipeptide utilization mediated by the ABC transporter DppBCDF and its substrate-binding proteins DppA1-A5 in Pseudomonas aeruginosa.
Pletzer D, Lafon C, Braun Y, Köhler T, Page MG, Mourez M, Weingart H
PLoS One 2014;9(10):e111311
PubMed ID: 25338022
Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach.
Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F
J Biomol Struct Dyn 2020 Sep;38(15):4508-4520
PubMed ID: 31631799