Pseudomonas aeruginosa PAO1, PA5325 (sphA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5325
Name
sphA
Replicon chromosome
Genomic location 5996036 - 5996986 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 4 transposon mutants in orthologs
CommentLoss of sphA leads to reduced survival in the murine lung and reduced resistance to the antimicrobial effect of sphingosine.

Cross-References

RefSeq NP_254012.1
GI 15600518
Affymetrix PA5325_at
DNASU PaCD00008300
Entrez 880923
GenBank AAG08710.1
INSDC AAG08710.1
NCBI Locus Tag PA5325
protein_id(GenBank) gb|AAG08710.1|AE004945_4|gnl|PseudoCAP|PA5325
TIGR NTL03PA05326
UniParc UPI00000C5FA6
UniProtKB Acc Q9HTN0
UniProtKB ID Q9HTN0_PSEAE
UniRef100 UniRef100_W0Z1I2
UniRef50 UniRef50_Q1I8G0
UniRef90 UniRef90_A6VED2

Product

Feature Type CDS
Coding Frame 1
Product Name
SphA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -7.41
Kyte-Doolittle Hydrophobicity Value -0.263
Molecular Weight (kDa) 35.5
Isoelectric Point (pI) 5.21

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Outer Membrane Class 2
sequence alignment evidence used in manual assertion
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 60 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003078 (1032 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5325
Search term: sphA
Search term: SphA

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Detection of host-derived sphingosine by Pseudomonas aeruginosa is important for survival in the murine lung.
LaBauve AE, Wargo MJ
PLoS Pathog 2014 Jan;10(1):e1003889
PubMed ID: 24465209
Crystal structure of a COG4313 outer membrane channel.
van den Berg B, Bhamidimarri SP, Winterhalter M
Sci Rep 2015 Jul 7;5:11927
PubMed ID: 26149193