Pseudomonas aeruginosa UCBPP-PA14, PA14_19490

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse

Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006)
GCF_000014625.1|latest
Locus Tag
PA14_19490
Name
Synonym: ahpC2
Replicon chromosome
Genomic location 1684916 - 1685554 (+ strand)
Transposon Mutants 1 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentReferred to as AhpC2 in PMID:34315109

Cross-References

RefSeq YP_789697.1
GI 116051470
Entrez 4385236
INSDC ABJ12706.1
NCBI Locus Tag PA14_19490
UniParc UPI00003960B2
UniProtKB Acc Q02QT9
UniProtKB ID Q02QT9_PSEAB
UniRef100 UniRef100_A0A071KYB2
UniRef50 UniRef50_Q54SE2
UniRef90 UniRef90_A0A024HLA3

Product

Feature Type CDS
Coding Frame 1
Product Name
1-Cys peroxiredoxin LsfA
Product Name Confidence: Class 1
Synonyms antioxidant protein
Evidence for Translation
Charge (pH 7) -5.83
Kyte-Doolittle Hydrophobicity Value -0.423
Molecular Weight (kDa) 24.2
Isoelectric Point (pI) 5.15

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7KUU OXIDOREDUCTASE 11/25/20 LsfA from P. aeruginosa, a 1-Cys Prx in Sulfonic acid form Pseudomonas aeruginosa (strain UCBPP-PA14) 2 X-RAY DIFFRACTION 100.0
6P0W OXIDOREDUCTASE 05/17/19 LsfA from P. aeruginosa, a 1-Cys Prx in reduced form Pseudomonas aeruginosa (strain UCBPP-PA14) 2.4 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database PseudoCAP
Category 1-Cys peroxyredoxin
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Mus musculus
Notes Mice infected with lsfA C45A mutant strain showed a reduced bacterial burden in all organs analyzed compared with wild-type PA14-infected
Infection model in vitro infection of J774 macrophages
Infection model Mouse acute pneumonia model (host
Reference 25329795

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 592 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002190 (528 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_19490

Human Homologs

Ensembl 110, assembly GRCh38.p14
peroxiredoxin 6 [Source:HGNC Symbol;Acc:HGNC:16753]
E-value: 3.3e-61
Percent Identity: 52.9

References

Peroxiredoxin AhpC1 protects Pseudomonas aeruginosa against the inflammatory oxidative burst and confers virulence.
Rocha LS, Silva BPD, Correia TML, Silva RPD, Meireles DA, Pereira R, Netto LES, Meotti FC, Queiroz RF
Redox Biol 2021 Oct;46:102075
PubMed ID: 34315109