Pseudomonas aeruginosa PAO1, PA0004 (gyrB)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 4275 - 6695 (+ strand)


RefSeq NP_064724.1
GI 15595202
Affymetrix PA0004_gyrB_at
Entrez 879230
GenBank AAG03394.1
INSDC AAG03394.1
NCBI Locus Tag PA0004
protein_id(GenBank) gb|AAG03394.1|AE004440_4|gnl|PseudoCAP|PA0004
UniParc UPI00000C4EE1
UniProtKB Acc Q9I7C2
UniRef100 UniRef100_Q9I7C2
UniRef50 UniRef50_P13364
UniRef90 UniRef90_Q9I7C2


Feature Type CDS
Coding Frame 1
Product Name
DNA gyrase subunit B
Product Name Confidence: Class 2
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -15.76
Kyte-Doolittle Hydrophobicity Value -0.411
Molecular Weight (kDa) 90.2
Isoelectric Point (pI) 5.74

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff E-value Percent Identity CARD Version
Mycobacterium tuberculosis gyrB mutant conferring resistance to fluoroquinolone Point mutation in Mycobacterium tuberculosis gyrB resulting in fluoroquinolone resistance ARO:3003459 protein variant model V340L Strict 0.0 0.485866 1.1.8

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 633 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000004 (561 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0004
Search term: gyrB

Human Homologs

Ensembl 86, assembly GRCh38.p7
topoisomerase (DNA) II alpha [Source:HGNC Symbol;Acc:HGNC:11989]
E-value: 1.8e-30
Percent Identity: 23.8


PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains.
Yamamoto S, Harayama S
Appl. Environ. Microbiol. 1995 Mar;61(3):1104-9
PubMed ID: 7793912
Type II topoisomerase mutations in ciprofloxacin-resistant strains of Pseudomonas aeruginosa.
Mouneimné H, Robert J, Jarlier V, Cambau E
Antimicrob. Agents Chemother. 1999 Jan;43(1):62-6
PubMed ID: 9869566
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994