Pseudomonas aeruginosa PAO1, PA0004 (gyrB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0004
Name
gyrB
Replicon chromosome
Genomic location 4275 - 6695 (+ strand)

Cross-References

RefSeq NP_064724.1
GI 15595202
Affymetrix PA0004_gyrB_at
Entrez 879230
GenBank AAG03394.1
INSDC AAG03394.1
NCBI Locus Tag PA0004
protein_id(GenBank) gb|AAG03394.1|AE004440_4|gnl|PseudoCAP|PA0004
TIGR NTL03PA00005
UniParc UPI00000C4EE1
UniProtKB Acc Q9I7C2
UniProtKB ID GYRB_PSEAE
UniRef100 UniRef100_Q9I7C2
UniRef50 UniRef50_P13364
UniRef90 UniRef90_Q9I7C2

Product

Feature Type CDS
Coding Frame 1
Product Name
DNA gyrase subunit B
Product Name Confidence: Class 2
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -15.76
Kyte-Doolittle Hydrophobicity Value -0.411
Molecular Weight (kDa) 90.2
Isoelectric Point (pI) 5.74

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff E-value Percent Identity CARD Version
Mycobacterium tuberculosis gyrB mutant conferring resistance to fluoroquinolone Point mutation in Mycobacterium tuberculosis gyrB resulting in fluoroquinolone resistance ARO:3003459 protein variant model V340L Strict 0.0 0.485866 1.1.8

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 633 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000004 (561 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0004
Search term: gyrB

Human Homologs

Ensembl 86, assembly GRCh38.p7
topoisomerase (DNA) II alpha [Source:HGNC Symbol;Acc:HGNC:11989]
E-value: 1.8e-30
Percent Identity: 23.8

References

PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains.
Yamamoto S, Harayama S
Appl. Environ. Microbiol. 1995 Mar;61(3):1104-9
PubMed ID: 7793912
Type II topoisomerase mutations in ciprofloxacin-resistant strains of Pseudomonas aeruginosa.
Mouneimné H, Robert J, Jarlier V, Cambau E
Antimicrob. Agents Chemother. 1999 Jan;43(1):62-6
PubMed ID: 9869566
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994