Pseudomonas aeruginosa PAO1, PA0024 (hemF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0024
Name
hemF
Replicon chromosome
Genomic location 25736 - 26653 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_248714.1
GI 15595222
Affymetrix PA0024_hemF_at
Entrez 880901
GenBank AAG03414.1
INSDC AAG03414.1
NCBI Locus Tag PA0024
protein_id(GenBank) gb|AAG03414.1|AE004442_1|gnl|PseudoCAP|PA0024
TIGR NTL03PA00025
UniParc UPI000012C4B5
UniProtKB Acc P43898
UniProtKB ID HEM6_PSEAE
UniRef100 UniRef100_Q02V57
UniRef50 UniRef50_P84155
UniRef90 UniRef90_A6UX86

Product

Feature Type CDS
Coding Frame 1
Product Name
coproporphyrinogen III oxidase, aerobic
Product Name Confidence: Class 2
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.53
Kyte-Doolittle Hydrophobicity Value -0.507
Molecular Weight (kDa) 34.8
Isoelectric Point (pI) 5.78

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 279 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000024 (573 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0024
Search term: hemF

Human Homologs

Ensembl 86, assembly GRCh38.p7
coproporphyrinogen oxidase [Source:HGNC Symbol;Acc:HGNC:2321]
E-value: 1.9e-73
Percent Identity: 44.4

Transposon Insertions in PAO1

Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_1_H6
Genomic Position 26647
Position in ORF 912(918)
Reference 15805499

Transposon Insertions in Orthologs

References

An oxygen-dependent coproporphyrinogen oxidase encoded by the hemF gene of Salmonella typhimurium.
Xu K, Elliott T.
J Bacteriol. 1993 Aug;175(16):4990-9.
PubMed ID: 8349542
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM.
Proteomics. 2009 Apr;9(7):1901-15.
PubMed ID: 19333994