Pseudomonas aeruginosa PAO1, PA0026 (plcB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0026
Name
plcB
Replicon chromosome
Genomic location 27646 - 28632 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_248716.1
GI 15595224
Affymetrix PA0026_at
DNASU PaCD00006015
Entrez 880904
GenBank AAG03416.1
INSDC AAG03416.1
NCBI Locus Tag PA0026
protein_id(GenBank) gb|AAG03416.1|AE004442_3|gnl|PseudoCAP|PA0026
TIGR NTL03PA00027
UniParc UPI00000C4EF0
UniProtKB Acc Q9I7A4
UniProtKB ID Q9I7A4_PSEAE
UniRef100 UniRef100_Q9I7A4
UniRef50 UniRef50_Q9I7A4
UniRef90 UniRef90_Q9I7A4

Product

Feature Type CDS
Coding Frame 1
Product Name
phospholipase C, PlcB
Product Name Confidence: Class 1
CommentPlcB is required for directed twitching motility up a gradient of phosphatidylcholine (PC), or phosphatidylethanolamine (PE) with specific type of fatty acid residues. PlcB can hyrolyse both PC and PE, and secreted via the Sec pathway. Proteins encoded by PA0027 & PA0028 are required for the proper folding (PA0027) of PlcB and to chaperone (PA0028) it through the inner and outer membrane.
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -10.94
Kyte-Doolittle Hydrophobicity Value -0.433
Molecular Weight (kDa) 36.7
Isoelectric Point (pI) 5.32

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 1
Unknown Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 6 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000026 (306 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0026
Search term: plcB

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 30624
Genomic Position 28288
Position in ORF 643(987)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 31030
Genomic Position 28141
Position in ORF 496(987)
Reference 14617778

Transposon Insertions in Orthologs

References

A novel extracellular phospholipase C of Pseudomonas aeruginosa is required for phospholipid chemotaxis.
Barker AP, Vasil AI, Filloux A, Ball G, Wilderman PJ, Vasil ML
Mol. Microbiol. 2004 Aug;53(4):1089-98
PubMed ID: 15306013