Pseudomonas aeruginosa PAO1, PA0026 (plcB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0026
Name
plcB
Replicon chromosome
Genomic location 27646 - 28632 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_248716.1
GI 15595224
Affymetrix PA0026_at
DNASU PaCD00006015
Entrez 880904
GenBank AAG03416.1
INSDC AAG03416.1
NCBI Locus Tag PA0026
protein_id(GenBank) gb|AAG03416.1|AE004442_3|gnl|PseudoCAP|PA0026
TIGR NTL03PA00027
UniParc UPI00000C4EF0
UniProtKB Acc Q9I7A4
UniProtKB ID Q9I7A4_PSEAE
UniRef100 UniRef100_Q9I7A4
UniRef50 UniRef50_Q9I7A4
UniRef90 UniRef90_Q9I7A4

Product

Feature Type CDS
Coding Frame 1
Product Name
phospholipase C, PlcB
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -10.94
Kyte-Doolittle Hydrophobicity Value -0.433
Molecular Weight (kDa) 36.7
Isoelectric Point (pI) 5.32

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Extracellular Class 1
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 6 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000026 (309 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0026
Search term: plcB

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 31030
Genomic Position 28141
Position in ORF 496(987)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 30624
Genomic Position 28288
Position in ORF 643(987)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_00300
Mutant ID PA14NR: 28999
Genomic Position 27675
Position in ORF 9(987)
Reference 16477005
Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_00300
Mutant ID PA14NR: 26176
Genomic Position 27829
Position in ORF 163(987)
Reference 16477005

References

A novel extracellular phospholipase C of Pseudomonas aeruginosa is required for phospholipid chemotaxis.
Barker AP, Vasil AI, Filloux A, Ball G, Wilderman PJ, Vasil ML.
Mol Microbiol. 2004 Aug;53(4):1089-98.
PubMed ID: 15306013