Pseudomonas aeruginosa PAO1, PA0035 (trpA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0035
Name
trpA
Replicon chromosome
Genomic location 37087 - 37893 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_248725.1
GI 15595233
Affymetrix PA0035_trpA_at
DNASU PaCD00006017
Entrez 879479
GenBank AAG03425.1
INSDC AAG03425.1
NCBI Locus Tag PA0035
protein_id(GenBank) gb|AAG03425.1|AE004443_3|gnl|PseudoCAP|PA0035
TIGR NTL03PA00036
UniParc UPI00001374E5
UniProtKB Acc P07344
UniProtKB ID TRPA_PSEAE
UniRef100 UniRef100_P07344
UniRef50 UniRef50_P07344
UniRef90 UniRef90_P07344

Product

Feature Type CDS
Coding Frame 1
Product Name
tryptophan synthase alpha chain
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -8.37
Kyte-Doolittle Hydrophobicity Value 0.061
Molecular Weight (kDa) 28.5
Isoelectric Point (pI) 5.00

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 581 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000035 (562 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0035
Search term: trpA

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 32053
Genomic Position 37437
Position in ORF 351(807)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 31715
Genomic Position 37512
Position in ORF 426(807)
Reference 14617778

Transposon Insertions in Orthologs

References

Nucleotide sequence of the genes for tryptophan synthase in Pseudomonas aeruginosa.
Hadero A, Crawford IP
Mol. Biol. Evol. 1986 May;3(3):191-204
PubMed ID: 3127651
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994