Pseudomonas aeruginosa PAO1, PA0036 (trpB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0036
Name
trpB
Replicon chromosome
Genomic location 37890 - 39098 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248726.1
GI 15595234
Affymetrix PA0036_trpB_at
Entrez 879212
GenBank AAG03426.1
INSDC AAG03426.1
NCBI Locus Tag PA0036
protein_id(GenBank) gb|AAG03426.1|AE004443_4|gnl|PseudoCAP|PA0036
TIGR NTL03PA00037
UniParc UPI0000137520
UniProtKB Acc P07345
UniProtKB ID TRPB_PSEAE
UniRef100 UniRef100_P07345
UniRef50 UniRef50_P07345
UniRef90 UniRef90_P07345

Product

Feature Type CDS
Coding Frame 1
Product Name
tryptophan synthase beta chain
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -7.08
Kyte-Doolittle Hydrophobicity Value -0.203
Molecular Weight (kDa) 43.7
Isoelectric Point (pI) 6.26

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 612 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000036 (574 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0036
Search term: trpB

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 32971
Genomic Position 37988
Position in ORF 99(1209)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 30248
Genomic Position 38525
Position in ORF 636(1209)
Reference 14617778
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 9255
Genomic Position 38846
Position in ORF 957(1209)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_00450
Mutant ID PA14NR: 43694
Genomic Position 38950
Position in ORF 1040(1209)
Reference 16477005

References

PSORTb 3.0: improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes.
Yu NY, Wagner JR, Laird MR, Melli G, Rey S, Lo R, Dao P, Sahinalp SC, Ester M, Foster LJ, Brinkman FS.
Bioinformatics. 2010 Jul 1;26(13):1608-15.
PubMed ID: 20472543
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM.
Proteomics. 2009 Apr;9(7):1901-15.
PubMed ID: 19333994