Pseudomonas aeruginosa PAO1, PA0067 (prlC)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Synonym: opdA
Replicon chromosome
Genomic location 78710 - 80755 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1


RefSeq NP_248757.1
GI 15595265
Affymetrix PA0067_prlC_at
Entrez 878482
GenBank AAG03457.1
INSDC AAG03457.1
NCBI Locus Tag PA0067
protein_id(GenBank) gb|AAG03457.1|AE004446_5|gnl|PseudoCAP|PA0067
UniParc UPI00000C4F14
UniProtKB Acc Q9I765
UniRef100 UniRef100_Q9I765
UniRef50 UniRef50_G8PY41
UniRef90 UniRef90_Q9I765


Feature Type CDS
Coding Frame 1
Product Name
oligopeptidase A
Product Name Confidence: Class 2
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -24.80
Kyte-Doolittle Hydrophobicity Value -0.253
Molecular Weight (kDa) 76.0
Isoelectric Point (pI) 4.91

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 335 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000066 (557 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0067
Search term: prlC
Search term: oligopeptidase A

Human Homologs

Ensembl 86, assembly GRCh38.p7
neurolysin [Source:HGNC Symbol;Acc:HGNC:16058]
E-value: 9.5e-81
Percent Identity: 29.9

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 3204
Genomic Position 79085
Position in ORF 376(2046)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Non-transcriptional fusion)
Mutant ID UBC: PAO1_lux_38_G5
Genomic Position 79244
Position in ORF 535(2046)
Reference 15805499
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 2001
Genomic Position 80192
Position in ORF 1483(2046)
Reference 14617778

Transposon Insertions in Orthologs


Escherichia coli prlC encodes an endopeptidase and is homologous to the Salmonella typhimurium opdA gene.
Conlin CA, Trun NJ, Silhavy TJ, Miller CG
J. Bacteriol. 1992 Sep;174(18):5881-7
PubMed ID: 1325967
Escherichia coli prlC gene encodes a trypsin-like proteinase regulating the cell cycle.
Jiang X, Zhang M, Ding Y, Yao J, Chen H, Zhu D, Muramatu M
J. Biochem. 1998 Nov;124(5):980-5
PubMed ID: 9792922
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994