Pseudomonas aeruginosa PAO1, PA0072 (tagS1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0072
Name
tagS1
Replicon chromosome
Genomic location 85085 - 86284 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248762.1
GI 15595270
Affymetrix PA0072_at
DNASU PaCD00006031
Entrez 879189
GenBank AAG03462.1
INSDC AAG03462.1
NCBI Locus Tag PA0072
protein_id(GenBank) gb|AAG03462.1|AE004446_10|gnl|PseudoCAP|PA0072
TIGR NTL03PA00073
UniParc UPI00000C4F19
UniProtKB Acc Q9I760
UniProtKB ID Q9I760_PSEAE
UniRef100 UniRef100_Q9I760
UniRef50 UniRef50_Q9I760
UniRef90 UniRef90_Q9I760

Product

Feature Type CDS
Coding Frame 1
Product Name
TagS1
Product Name Confidence: Class 2
Evidence for Translation
Charge (pH 7) 6.88
Kyte-Doolittle Hydrophobicity Value 0.430
Molecular Weight (kDa) 42.2
Isoelectric Point (pI) 9.57

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database VFDB : VF0334
Category Hcp secretion island-1 encoded type VI secretion system (H-T6SS)
Evidence ECO:0000305
curator inference used in manual assertion
Host Organism
Notes
Reference 16763151

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 35 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000071 (429 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0072
Search term: tagS1
Search term: TagS1

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 38190
Genomic Position 85958
Position in ORF 874(1200)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 55261
Genomic Position 85578
Position in ORF 494(1200)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_00850
Mutant ID PA14NR: 41307
Genomic Position 83263
Position in ORF 534(1200)
Reference 16477005

References

A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
Hood RD, Singh P, Hsu F, Guvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD.
Cell Host Microbe. 2010 Jan 21;7(1):25-37.
PubMed ID: 20114026
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS.
Microbiology. 2007 Aug;153(Pt 8):2689-99.
PubMed ID: 17660433