Pseudomonas aeruginosa PAO1, PA0074 (ppkA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0074
Name
ppkA
Synonym: tagE1
Replicon chromosome
Genomic location 87000 - 90098 (- strand)
Transposon Mutants 9 transposon mutants in PAO1

Cross-References

RefSeq NP_248764.1
GI 15595272
Affymetrix PA0074_ppkA_at
Entrez 879361
GenBank AAG03464.1
INSDC AAG03464.1
NCBI Locus Tag PA0074
protein_id(GenBank) gb|AAG03464.1|AE004446_12|gnl|PseudoCAP|PA0074
TIGR NTL03PA00075
UniParc UPI00000C4F1B
UniProtKB Acc Q9I758
UniProtKB ID Q9I758_PSEAE
UniRef100 UniRef100_Q9I758
UniRef50 UniRef50_Q9I758
UniRef90 UniRef90_Q9I758

Product

Feature Type CDS
Coding Frame 1
Product Name
serine/threonine protein kinase PpkA
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (110.02 kDa, PMID:21751344)
Charge (pH 7) -15.36
Kyte-Doolittle Hydrophobicity Value -0.244
Molecular Weight (kDa) 110.0
Isoelectric Point (pI) 5.15

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database PseudoCAP
Category Regulation of Type VI Secretion
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Mus musculus
Notes Category of Regulation of Type VI secretion based on PMID: 21880152 and PMID: 19400797
Infection model neutropenic mice
Reference 9852028
Source 2
Database VFDB : VF0334
Category Hcp secretion island-1 encoded type VI secretion system (H-T6SS)
Evidence ECO:0000305
curator inference used in manual assertion
Host Organism
Notes
Reference 16763151

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 463 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000073 (1415 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0074
Search term: ppkA

Human Homologs

Ensembl 86, assembly GRCh38.p7
pregnancy up-regulated nonubiquitous CaM kinase [Source:HGNC Symbol;Acc:HGNC:13415]
E-value: 4.1e-27
Percent Identity: 34.2

Transposon Insertions in PAO1

Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_15_D7
Genomic Position 87177
Position in ORF 178(3099)
Reference 15805499
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_3_B6
Genomic Position 87661
Position in ORF 662(3099)
Reference 15805499
Type ISphoA/hah insertion site
Mutant ID UWGC: 32179
Genomic Position 88358
Position in ORF 1359(3099)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_3_B9
Genomic Position 87702
Position in ORF 703(3099)
Reference 15805499
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_3_A6
Genomic Position 87794
Position in ORF 795(3099)
Reference 15805499
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_2_C7
Genomic Position 87774
Position in ORF 775(3099)
Reference 15805499
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_3_C6
Genomic Position 87758
Position in ORF 759(3099)
Reference 15805499
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_1_F3
Genomic Position 87661
Position in ORF 662(3099)
Reference 15805499
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 31649
Genomic Position 88475
Position in ORF 1476(3099)
Reference 14617778

Transposon Insertions in Orthologs

References

A novel serine/threonine protein kinase homologue of Pseudomonas aeruginosa is specifically inducible within the host infection site and is required for full virulence in neutropenic mice.
Wang J, Li C, Yang H, Mushegian A, Jin S
J. Bacteriol. 1998 Dec;180(24):6764-8
PubMed ID: 9852028
Functional characterization of a serine/threonine protein kinase of Pseudomonas aeruginosa.
Motley ST, Lory S
Infect. Immun. 1999 Oct;67(10):5386-94
PubMed ID: 10496921
A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus.
Mougous JD, Cuff ME, Raunser S, Shen A, Zhou M, Gifford CA, Goodman AL, Joachimiak G, Ordoñez CL, Lory S, Walz T, Joachimiak A, Mekalanos JJ
Science 2006 Jun;312(5779):1526-30
PubMed ID: 16763151
A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD
Cell Host Microbe 2010 Jan;7(1):25-37
PubMed ID: 20114026
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology (Reading, Engl.) 2007 Aug;153(Pt 8):2689-99
PubMed ID: 17660433
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344