Pseudomonas aeruginosa PAO1, PA0079 (tssK1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0079
Name
tssK1
Replicon chromosome
Genomic location 96404 - 97738 (- strand)
Transposon Mutants 6 transposon mutants in PAO1

Cross-References

RefSeq NP_248769.1
GI 15595277
Affymetrix PA0079_at
Entrez 879382
GenBank AAG03469.1
INSDC AAG03469.1
NCBI Locus Tag PA0079
protein_id(GenBank) gb|AAG03469.1|AE004447_2|gnl|PseudoCAP|PA0079
TIGR NTL03PA00080
UniParc UPI00000C4F20
UniProtKB Acc Q9I753
UniProtKB ID Q9I753_PSEAE
UniRef100 UniRef100_Q9I753
UniRef50 UniRef50_Q4K3P2
UniRef90 UniRef90_Q9I753

Product

Feature Type CDS
Coding Frame 1
Product Name
TssK1
Product Name Confidence: Class 2
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.92
Kyte-Doolittle Hydrophobicity Value -0.021
Molecular Weight (kDa) 48.3
Isoelectric Point (pI) 6.06

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database VFDB : VF0334
Category Hcp secretion island-1 encoded type VI secretion system (H-T6SS)
Evidence ECO:0000305
curator inference used in manual assertion
Host Organism
Notes
Reference 16763151

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 109 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000078 (894 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0079
Search term: tssK1
Search term: TssK1

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 390
Genomic Position 96625
Position in ORF 222(1335)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Non-transcriptional fusion)
Mutant ID UBC: PAO1_lux_42_E12
Genomic Position 96713
Position in ORF 310(1335)
Reference 15805499
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_25_B9
Genomic Position 96469
Position in ORF 66(1335)
Reference 15805499
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 40338
Genomic Position 96950
Position in ORF 547(1335)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_66_G7
Genomic Position 97635
Position in ORF 1232(1335)
Reference 15805499
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_12_G3
Genomic Position 97269
Position in ORF 866(1335)
Reference 15805499

Transposon Insertions in Orthologs

References

A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD
Cell Host Microbe 2010 Jan;7(1):25-37
PubMed ID: 20114026
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology (Reading, Engl.) 2007 Aug;153(Pt 8):2689-99
PubMed ID: 17660433