Pseudomonas aeruginosa PAO1, PA0087 (tssE1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0087
Name
tssE1
Replicon chromosome
Genomic location 104853 - 105362 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1

Cross-References

RefSeq NP_248777.1
GI 15595285
Affymetrix PA0087_at
DNASU PaCD00006039
Entrez 879495
GenBank AAG03477.1
INSDC AAG03477.1
NCBI Locus Tag PA0087
protein_id(GenBank) gb|AAG03477.1|AE004447_10|gnl|PseudoCAP|PA0087
TIGR NTL03PA00088
UniParc UPI00000C4F28
UniProtKB Acc Q9I745
UniProtKB ID Q9I745_PSEAE
UniRef100 UniRef100_W0YM19
UniRef50 UniRef50_Q02UZ2
UniRef90 UniRef90_Q02UZ2

Product

Feature Type CDS
Coding Frame 1
Product Name
TssE1
Product Name Confidence: Class 2
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.80
Kyte-Doolittle Hydrophobicity Value -0.178
Molecular Weight (kDa) 18.7
Isoelectric Point (pI) 5.27

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database VFDB : VF0334
Category Hcp secretion island-1 encoded type VI secretion system (H-T6SS)
Evidence ECO:0000305
curator inference used in manual assertion
Host Organism
Notes
Reference 16763151

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 64 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000086 (851 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0087
Search term: tssE1
Search term: TssE1

Transposon Insertions in PAO1

Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 39666
Genomic Position 105268
Position in ORF 416(510)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 49678
Genomic Position 105055
Position in ORF 203(510)
Reference 14617778
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 39798
Genomic Position 105268
Position in ORF 416(510)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_98_B2
Genomic Position 105018
Position in ORF 166(510)
Reference 15805499

Transposon Insertions in Orthologs

References

A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD
Cell Host Microbe 2010 Jan;7(1):25-37
PubMed ID: 20114026
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology (Reading, Engl.) 2007 Aug;153(Pt 8):2689-99
PubMed ID: 17660433