Pseudomonas aeruginosa PAO1, PA0148

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0148
Name
Synonym: add
Replicon chromosome
Genomic location 168213 - 169163 (+ strand)
Transposon Mutants 6 transposon mutants in PAO1

Cross-References

RefSeq NP_248838.1
GI 15595346
Affymetrix PA0148_at
Entrez 879249
GenBank AAG03538.1
INSDC AAG03538.1
NCBI Locus Tag PA0148
protein_id(GenBank) gb|AAG03538.1|AE004453_1|gnl|PseudoCAP|PA0148
TIGR NTL03PA00149
UniParc UPI0000125510
UniProtKB Acc Q9I6Y4
UniProtKB ID ADE_PSEAE
UniRef100 UniRef100_B7V265
UniRef50 UniRef50_A6UXT7
UniRef90 UniRef90_A6UXT7

Product

Feature Type CDS
Coding Frame 1
Product Name
adenine deaminase
Product Name Confidence: Class 3
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -14.43
Kyte-Doolittle Hydrophobicity Value -0.275
Molecular Weight (kDa) 36.2
Isoelectric Point (pI) 4.87

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3PAN HYDROLASE 10/19/10 The crystal structure of adenosine deaminase with hypoxanthine bound from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2.627 X-RAY DIFFRACTION 99.7
3PBM HYDROLASE 10/20/10 The crystal structure of adenosine deaminase in complex with chloropurine from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2.591 X-RAY DIFFRACTION 99.7
3OU8 HYDROLASE 09/14/10 The crystal structure of adenosine deaminase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2.51 X-RAY DIFFRACTION 99.7
3PAO HYDROLASE 10/19/10 The crystal structure of adenosine deaminase with adenine bound from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2.493 X-RAY DIFFRACTION 99.7

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 254 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000146 (557 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0148
Search term: adenine deaminase

Transposon Insertions in PAO1

Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 40683
Genomic Position 168471
Position in ORF 259(951)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_67_E5
Genomic Position 168586
Position in ORF 374(951)
Reference 15805499
Type mini-Tn5 lux transposon insertion site (Non-transcriptional fusion)
Mutant ID UBC: PAO1_lux_44_A3
Genomic Position 169010
Position in ORF 798(951)
Reference 15805499
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 2253
Genomic Position 169056
Position in ORF 844(951)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_32_E10
Genomic Position 168853
Position in ORF 641(951)
Reference 15805499
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 35276
Genomic Position 168840
Position in ORF 628(951)
Reference 14617778

Transposon Insertions in Orthologs

References

Deduced amino acid sequence of Escherichia coli adenosine deaminase reveals evolutionarily conserved amino acid residues: implications for catalytic function.
Chang ZY, Nygaard P, Chinault AC, Kellems RE
Biochemistry 1991 Feb;30(8):2273-80
PubMed ID: 1998686
Pa0148 from Pseudomonas aeruginosa catalyzes the deamination of adenine.
Goble AM, Zhang Z, Sauder JM, Burley SK, Swaminathan S, Raushel FM
Biochemistry 2011 Aug;50(30):6589-97
PubMed ID: 21710971