Pseudomonas aeruginosa PAO1, PA0156 (triA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0156
Name
triA
Synonym: triA
Replicon chromosome
Genomic location 177307 - 178458 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248846.1
GI 15595354
Affymetrix PA0156_at
Entrez 882149
GenBank AAG03546.1
INSDC AAG03546.1
NCBI Locus Tag PA0156
protein_id(GenBank) gb|AAG03546.1|AE004453_9|gnl|PseudoCAP|PA0156
TIGR NTL03PA00157
UniParc UPI00000C4F6B
UniProtKB Acc Q9I6X6
UniProtKB ID Q9I6X6_PSEAE
UniRef100 UniRef100_U9HZG4
UniRef50 UniRef50_Q4KK51
UniRef90 UniRef90_W0YM83

Product

Feature Type CDS
Coding Frame 1
Product Name
Resistance-Nodulation-Cell Division (RND) triclosan efflux membrane fusion protein, TriA
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.61
Kyte-Doolittle Hydrophobicity Value -0.137
Molecular Weight (kDa) 40.4
Isoelectric Point (pI) 6.53

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown (This protein may have multiple localization sites) Class 3
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 422 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000154 (1062 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0156
Search term: triA

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 32948
Genomic Position 177616
Position in ORF 310(1152)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 55138
Genomic Position 177992
Position in ORF 686(1152)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_01940
Mutant ID PA14NR: 47877
Genomic Position 176367
Position in ORF 201(1152)
Reference 16477005

References

Identification and characterization of TriABC-OpmH, a triclosan efflux pump of Pseudomonas aeruginosa requiring two membrane fusion proteins.
Mima T, Joshi S, Gomez-Escalada M, Schweizer HP.
J Bacteriol. 2007 Nov;189(21):7600-9.
PubMed ID: 17720796