Pseudomonas aeruginosa PAO1, PA0162 (opdC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0162
Name
opdC
Synonym: opdC
Replicon chromosome
Genomic location 184594 - 185928 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_248852.1
GI 15595360
Affymetrix PA0162_at
Entrez 879518
GenBank AAG03552.1
INSDC AAG03552.1
NCBI Locus Tag PA0162
protein_id(GenBank) gb|AAG03552.1|AE004454_4|gnl|PseudoCAP|PA0162
TIGR NTL03PA00163
UniParc UPI00000C4F71
UniProtKB Acc Q9I6X0
UniProtKB ID Q9I6X0_PSEAE
UniRef100 UniRef100_Q9I6X0
UniRef50 UniRef50_V6A7C8
UniRef90 UniRef90_V6A7C8

Product

Feature Type CDS
Coding Frame 1
Product Name
histidine porin OpdC
Product Name Confidence: Class 1
Evidence for Translation
Detected using LC-MS/MS (48.86 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -15.39
Kyte-Doolittle Hydrophobicity Value -0.488
Molecular Weight (kDa) 48.9
Isoelectric Point (pI) 4.79

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Outer Membrane Class 2
Outer Membrane Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3SY9 membrane protein 07/16/11 Crystal structure of Pseudomonas aeruginosa OccD2 (OpdC) PSEUDOMONAS AERUGINOSA 2.8 X-RAY DIFFRACTION 99.8

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 24 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000160 (459 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0162
Search term: opdC

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 51365
Genomic Position 185324
Position in ORF 731(1335)
Reference 14617778
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 15016
Genomic Position 185470
Position in ORF 877(1335)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_02020
Mutant ID PA14NR: 45464
Genomic Position 184052
Position in ORF 599(1335)
Reference 16477005
Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_02020
Mutant ID PA14NR: 46968
Genomic Position 184653
Position in ORF 1200(1335)
Reference 16477005

References

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM.
Proteomics. 2009 Apr;9(7):1901-15.
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS.
Proteomics. 2011 Aug;11(16):3424-9.
PubMed ID: 21751344