Pseudomonas aeruginosa PAO1, PA0208 (mdcA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0208
Name
mdcA
Replicon chromosome
Genomic location 237232 - 238896 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_248899.1
GI 15595405
Affymetrix PA0208_mdcA_at
Entrez 879322
GenBank AAG03597.1
INSDC AAG03597.1
NCBI Locus Tag PA0208
protein_id(GenBank) gb|AAG03597.1|AE004459_1|gnl|PseudoCAP|PA0208
TIGR NTL03PA00209
UniParc UPI00000C4F98
UniProtKB Acc Q9I6T0
UniProtKB ID Q9I6T0_PSEAE
UniRef100 UniRef100_Q9I6T0
UniRef50 UniRef50_O06924
UniRef90 UniRef90_Q9I6T0

Product

Feature Type CDS
Coding Frame 1
Product Name
malonate decarboxylase alpha subunit
Product Name Confidence: Class 2
Evidence for Translation
Charge (pH 7) -5.21
Kyte-Doolittle Hydrophobicity Value -0.182
Molecular Weight (kDa) 61.4
Isoelectric Point (pI) 6.42

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 59 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000207 (503 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0208
Search term: mdcA

Transposon Insertions in PAO1

Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 1786
Genomic Position 238177
Position in ORF 946(1665)
Reference 14617778
Type ISlacZ/hah insertion site
Mutant ID UWGC: 6846
Genomic Position 237927
Position in ORF 696(1665)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_02550
Mutant ID PA14NR: 29044
Genomic Position 228044
Position in ORF 662(1665)
Reference 16477005
Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_02550
Mutant ID PA14NR: 32272
Genomic Position 228792
Position in ORF 1410(1665)
Reference 16477005

References

Sequence of a gene cluster from Klebsiella pneumoniae encoding malonate decarboxylase and expression of the enzyme in Escherichia coli.
Hoenke S, Schmid M, Dimroth P.
Eur J Biochem. 1997 Jun 1;246(2):530-8.
PubMed ID: 9208947