Pseudomonas aeruginosa PAO1, PA0285 (pipA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0285
Name
pipA
Replicon chromosome
Genomic location 318312 - 320594 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248976.1
GI 15595482
Affymetrix PA0285_at
Entrez 879674
GenBank AAG03674.1
INSDC AAG03674.1
NCBI Locus Tag PA0285
protein_id(GenBank) gb|AAG03674.1|AE004466_8|gnl|PseudoCAP|PA0285
TIGR NTL03PA00286
UniParc UPI00000C4FE1
UniProtKB Acc Q9I6K5
UniProtKB ID Q9I6K5_PSEAE
UniRef100 UniRef100_Q9I6K5
UniRef50 UniRef50_Q4KK97
UniRef90 UniRef90_Q9I6K5

Product

Feature Type CDS
Coding Frame 1
Product Name
cyclic di-GMP phosphodiesterase, PipA
Product Name Confidence: Class 1
Synonyms putative phosphodiesterase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -12.43
Kyte-Doolittle Hydrophobicity Value -0.275
Molecular Weight (kDa) 86.4
Isoelectric Point (pI) 6.31

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 536 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000282 (516 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0285
Search term: pipA

Human Homologs

References

Diguanylate Cyclases and Phosphodiesterases Required for Basal-Level c-di-GMP in Pseudomonas aeruginosa as Revealed by Systematic Phylogenetic and Transcriptomic Analyses.
Wei Q, Leclercq S, Bhasme P, Xu A, Zhu B, Zhang Y, Zhang M, Wang S, Ma LZ
Appl Environ Microbiol 2019 Nov 1;85(21)
PubMed ID: 31444209
The c-di-GMP Phosphodiesterase PipA (PA0285) Regulates Autoaggregation and Pf4 Bacteriophage Production in Pseudomonas aeruginosa PAO1.
Cai YM, Yu KW, Liu JH, Cai Z, Zhou ZH, Liu Y, Wang TF, Yang L
Appl Environ Microbiol 2022 Jun 28;88(12):e0003922
PubMed ID: 35638845