Pseudomonas aeruginosa PAO1, PA0296 (spuI)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0296
Name
spuI
Replicon chromosome
Genomic location 333079 - 334455 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_248987.1
GI 15595493
Affymetrix PA0296_s_at
Entrez 880840
GenBank AAG03685.1
INSDC AAG03685.1
NCBI Locus Tag PA0296
protein_id(GenBank) gb|AAG03685.1|AE004467_7|gnl|PseudoCAP|PA0296
TIGR NTL03PA00297
UniParc UPI00000C4FEB
UniProtKB Acc Q9I6J5
UniProtKB ID Q9I6J5_PSEAE
UniRef100 UniRef100_A0A072ZGE6
UniRef50 UniRef50_G8Q4R6
UniRef90 UniRef90_A0A072ZGE6

Product

Feature Type CDS
Coding Frame 1
Product Name
Glutamylpolyamine synthetase
Product Name Confidence: Class 1
Synonyms pauA1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -25.76
Kyte-Doolittle Hydrophobicity Value -0.242
Molecular Weight (kDa) 50.9
Isoelectric Point (pI) 4.56

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 593 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000293 (728 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0296
Search term: spuI

Human Homologs

References

Functional characterization of seven γ-Glutamylpolyamine synthetase genes and the bauRABCD locus for polyamine and β-Alanine utilization in Pseudomonas aeruginosa PAO1.
Yao X, He W, Lu CD
J. Bacteriol. 2011 Aug;193(15):3923-30
PubMed ID: 21622750