Pseudomonas aeruginosa PAO1, PA0302 (spuF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0302
Name
spuF
Synonym: potG
Replicon chromosome
Genomic location 341111 - 342265 (+ strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_248993.1
GI 15595499
Affymetrix PA0302_potG_at
DNASU PaCD00006131
Entrez 877636
GenBank AAG03691.1
INSDC AAG03691.1
NCBI Locus Tag PA0302
protein_id(GenBank) gb|AAG03691.1|AE004468_3|gnl|PseudoCAP|PA0302
TIGR NTL03PA00303
UniParc UPI00000C4FF1
UniProtKB Acc Q9I6I9
UniProtKB ID Q9I6I9_PSEAE
UniRef100 UniRef100_W0YL67
UniRef50 UniRef50_P31134
UniRef90 UniRef90_A6UYA3

Product

Feature Type CDS
Coding Frame 1
Product Name
polyamine transport protein PotG
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected using LC-MS/MS (42.78 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.47
Kyte-Doolittle Hydrophobicity Value -0.140
Molecular Weight (kDa) 42.8
Isoelectric Point (pI) 6.44

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 669 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000299 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0302
Search term: spuF

Human Homologs

Ensembl 110, assembly GRCh38.p14
ATP binding cassette subfamily B member 10 [Source:HGNC Symbol;Acc:HGNC:41]
E-value: 1.2e-21
Percent Identity: 36.2

References

Characteristics of the operon for a putrescine transport system that maps at 19 minutes on the Escherichia coli chromosome.
Pistocchi R, Kashiwagi K, Miyamoto S, Nukui E, Sadakata Y, Kobayashi H, Igarashi K
J Biol Chem 1993 Jan 5;268(1):146-52
PubMed ID: 8416922
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344