Pseudomonas aeruginosa PAO1, PA0305 (hacB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0305
Name
hacB
Replicon chromosome
Genomic location 344303 - 346690 (- strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_248996.1
GI 15595502
Affymetrix PA0305_at
Entrez 877593
GenBank AAG03694.1
INSDC AAG03694.1
NCBI Locus Tag PA0305
protein_id(GenBank) gb|AAG03694.1|AE004468_6|gnl|PseudoCAP|PA0305
TIGR NTL03PA00306
UniParc UPI00000C4FF4
UniProtKB Acc Q9I6I6
UniProtKB ID Q9I6I6_PSEAE
UniRef100 UniRef100_Q9I6I6
UniRef50 UniRef50_D7I6B0
UniRef90 UniRef90_Q9I6I6

Product

Feature Type CDS
Coding Frame 1
Product Name
acylhomoserine lactone acylase B
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -15.67
Kyte-Doolittle Hydrophobicity Value -0.364
Molecular Weight (kDa) 87.5
Isoelectric Point (pI) 5.68

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 225 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000302 (493 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0305
Search term: hacB

Human Homologs

References

Chemical modification of serine at the active site of penicillin acylase from Kluyvera citrophila.
Martín J, Slade A, Aitken A, Arche R, Virden R
Biochem J 1991 Dec 15;280 ( Pt 3)(Pt 3):659-62
PubMed ID: 1764029
PA0305 of Pseudomonas aeruginosa is a quorum quenching acylhomoserine lactone acylase belonging to the Ntn hydrolase superfamily.
Wahjudi M, Papaioannou E, Hendrawati O, van Assen AHG, van Merkerk R, Cool RH, Poelarends GJ, Quax WJ
Microbiology (Reading) 2011 Jul;157(Pt 7):2042-2055
PubMed ID: 21372094