Pseudomonas aeruginosa PAO1, PA0411 (pilJ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0411
Name
pilJ
Replicon chromosome
Genomic location 451130 - 453178 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249102.1
GI 15595608
Affymetrix PA0411_pilJ_at
Entrez 878180
GenBank AAG03800.1
INSDC AAG03800.1
NCBI Locus Tag PA0411
protein_id(GenBank) gb|AAG03800.1|AE004478_5|gnl|PseudoCAP|PA0411
TIGR NTL03PA00412
UniParc UPI0000131ACC
UniProtKB Acc P42257
UniProtKB ID PILJ_PSEAE
UniRef100 UniRef100_P42257
UniRef50 UniRef50_P42257
UniRef90 UniRef90_P42257

Product

Feature Type CDS
Coding Frame 1
Product Name
twitching motility protein PilJ
Product Name Confidence: Class 1
Synonyms type 4 fimbrial biogenesis protein PilJ
Evidence for Translation
Detected using LC-MS/MS (72.53 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -33.25
Kyte-Doolittle Hydrophobicity Value -0.060
Molecular Weight (kDa) 72.5
Isoelectric Point (pI) 4.37

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Outer Membrane Vesicle Class 1
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7ZJR SIGNALING PROTEIN 04/11/22 X-ray structure of the periplasmic region of PilJ from P. aeruginosa Pseudomonas aeruginosa PAO1 2.50312 X-RAY DIFFRACTION 99.6

Virulence Evidence

Source 1
Database VFDB : VF0082
Category Type IV pili twitching motility related proteins
Evidence
Host Organism
Notes
Reference 9224879

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 397 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000402 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0411
Search term: pilJ

Human Homologs

References

Characterization of a Pseudomonas aeruginosa gene cluster involved in pilus biosynthesis and twitching motility: sequence similarity to the chemotaxis proteins of enterics and the gliding bacterium Myxococcus xanthus.
Darzins A
Mol Microbiol 1994 Jan;11(1):137-53
PubMed ID: 7908398
The molecular genetics of type-4 fimbriae in Pseudomonas aeruginosa--a review.
Mattick JS, Whitchurch CB, Alm RA
Gene 1996 Nov 7;179(1):147-55
PubMed ID: 8955641
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344