Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA0432
|
Name |
sahH
Synonym: ahcY |
Replicon | chromosome |
Genomic location | 482706 - 484115 (- strand) |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
RefSeq | NP_249123.1 |
GI | 15595629 |
Affymetrix | PA0432_sahH_at |
Entrez | 877746 |
GenBank | AAG03821.1 |
INSDC | AAG03821.1 |
NCBI Locus Tag | PA0432 |
protein_id(GenBank) | gb|AAG03821.1|AE004480_5|gnl|PseudoCAP|PA0432 |
TIGR | NTL03PA00433 |
UniParc | UPI0000135502 |
UniProtKB Acc | Q9I685 |
UniProtKB ID | SAHH_PSEAE |
UniRef100 | UniRef100_B7V419 |
UniRef50 | UniRef50_Q4K4H7 |
UniRef90 | UniRef90_A4XPF3 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
S-adenosyl-L-homocysteine hydrolase
|
Synonyms |
adenosylhomocysteinase (adohcyase) |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
|
Charge (pH 7) | -7.34 |
Kyte-Doolittle Hydrophobicity Value | -0.189 |
Molecular Weight (kDa) | 51.4 |
Isoelectric Point (pI) | 5.96 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
6F3P | HYDROLASE | 11/28/17 | Crystal structure of S-adenosyl-L-homocysteine hydrolase from Pseudomonas aeruginosa in complex with 3'-deoxyadenosine and K+ cation | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 1.35 | X-RAY DIFFRACTION | 100.0 |
7ZD4 | HYDROLASE | 03/29/22 | Crystal structure of Pseudomonas aeruginosa S-adenosyl-L-homocysteine hydrolase soaked with Cu+ ions | Pseudomonas aeruginosa PAO1 | 2.14 | X-RAY DIFFRACTION | 100.0 |
7ZD1 | HYDROLASE | 03/29/22 | Crystal structure of Pseudomonas aeruginosa S-adenosyl-L-homocysteine hydrolase inhibited by Hg2+ ions | Pseudomonas aeruginosa PAO1 | 1.56 | X-RAY DIFFRACTION | 100.0 |
7ZD2 | HYDROLASE | 03/29/22 | Crystal structure of Pseudomonas aeruginosa S-adenosyl-L-homocysteine hydrolase inhibited by Co2+ ions. | Pseudomonas aeruginosa PAO1 | 2.16 | X-RAY DIFFRACTION | 100.0 |
6F3O | HYDROLASE | 11/28/17 | Crystal structure of S-adenosyl-L-homocysteine hydrolase from Pseudomonas aeruginosa complexed with adenine, K+ and Zn2+ cations | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 1.75 | X-RAY DIFFRACTION | 100.0 |
7ZD0 | HYDROLASE | 03/29/22 | Crystal structure of Pseudomonas aeruginosa S-adenosyl-L-homocysteine hydrolase inhibited by Cd2+ ions | Pseudomonas aeruginosa PAO1 | 1.87 | X-RAY DIFFRACTION | 100.0 |
7ZD3 | HYDROLASE | 03/29/22 | Crystal structure of Pseudomonas aeruginosa S-adenosyl-L-homocysteine hydrolase inhibited by Zn2+ ions | Pseudomonas aeruginosa PAO1 | 1.9 | X-RAY DIFFRACTION | 100.0 |
6F3N | HYDROLASE | 11/28/17 | Crystal structure of S-adenosyl-L-homocysteine hydrolase from Pseudomonas aeruginosa cocrystallized with SAH in the presence of K+ and Zn2+ cations | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 1.85 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 500 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000423 (537 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA0432
Search term: sahH
Search term: S-adenosyl-L-homocysteine hydrolase
|
Ensembl
110, assembly
GRCh38.p14
|
adenosylhomocysteinase [Source:HGNC Symbol;Acc:HGNC:343]
E-value:
1.3e-134
Percent Identity:
56.2
|
Amino acid sequence of S-adenosyl-L-homocysteine hydrolase from rat liver as derived from the cDNA sequence.
Ogawa H, Gomi T, Mueckler MM, Fujioka M, Backlund PS Jr, Aksamit RR, Unson CG, Cantoni GL
Proc Natl Acad Sci U S A 1987 Feb;84(3):719-23
PubMed ID: 3027698
|
Isozyme and DNA analysis of human S-adenosyl-L-homocysteine hydrolase (AHCY).
Arredondo-Vega FX, Charlton JA, Edwards YH, Hopkinson DA, Whitehouse DB
Ann Hum Genet 1989 May;53(2):157-67
PubMed ID: 2574561
|
Mutational and nucleotide sequence analysis of S-adenosyl-L-homocysteine hydrolase from Rhodobacter capsulatus.
Sganga MW, Aksamit RR, Cantoni GL, Bauer CE
Proc Natl Acad Sci U S A 1992 Jul 15;89(14):6328-32
PubMed ID: 1631127
|
S-adenosylhomocysteine hydrolase from Acinetobacter calcoaceticus: purification and partial characterization.
Franceschini N, Impagnatiello A, Oratore A, Strom R, Bozzi A
Biochem Mol Biol Int 1993 Aug;30(6):1081-92
PubMed ID: 8220254
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|