Pseudomonas aeruginosa PAO1, PA0579 (rpsU)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0579
Name
rpsU
Replicon chromosome
Genomic location 638900 - 639115 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249270.1
GI 15595776
Affymetrix PA0579_rpsU_at
DNASU PaCD00006253
Entrez 880307
GenBank AAG03968.1
INSDC AAG03968.1
NCBI Locus Tag PA0579
protein_id(GenBank) gb|AAG03968.1|AE004494_4|gnl|PseudoCAP|PA0579
TIGR NTL03PA00580
UniParc UPI0000134E83
UniProtKB Acc Q9I5V8
UniProtKB ID RS21_PSEAE
UniRef100 UniRef100_C1DIY4
UniRef50 UniRef50_B4RY34
UniRef90 UniRef90_C1DIY4

Product

Feature Type CDS
Coding Frame 1
Product Name
30S ribosomal protein S21
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 13.19
Kyte-Doolittle Hydrophobicity Value -1.193
Molecular Weight (kDa) 8.5
Isoelectric Point (pI) 11.43

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7UNR RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPG RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from a clinical isolate Pseudomonas aeruginosa 3.34 ELECTRON MICROSCOPY 98.4
7UNW RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.6 ELECTRON MICROSCOPY 100.0
6SPE RIBOSOME 09/01/19 Pseudomonas aeruginosa 30s ribosome from a clinical isolate Pseudomonas aeruginosa 3.6 ELECTRON MICROSCOPY 98.4
7UNV RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.7 ELECTRON MICROSCOPY 100.0
7UNU RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 358 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000562 (493 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0579
Search term: rpsU

Human Homologs

References

The function of ribosomal protein S21 in protein synthesis.
Van Duin J, Wijnands R
Eur J Biochem 1981 Sep 1;118(3):615-9
PubMed ID: 7028483
The operon that encodes the sigma subunit of RNA polymerase also encodes ribosomal protein S21 and DNA primase in E. coli K12.
Burton ZF, Gross CA, Watanabe KK, Burgess RR
Cell 1983 Feb;32(2):335-49
PubMed ID: 6186393
Regulation of the rpsU-dnaG-rpoD macromolecular synthesis operon and the initiation of DNA replication in Escherichia coli K-12.
Lupski JR, Smiley BL, Godson GN
Mol Gen Genet 1983;189(1):48-57
PubMed ID: 6222240
Nucleotide sequence of the rpsU-dnaG-rpoD operon from Salmonella typhimurium and a comparison of this sequence with the homologous operon of Escherichia coli.
Erickson BD, Burton ZF, Watanabe KK, Burgess RR
Gene 1985;40(1):67-78
PubMed ID: 3005129