Pseudomonas aeruginosa PAO1, PA0616

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0616
Name
Replicon chromosome
Genomic location 676373 - 676930 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249307.1
GI 15595813
Affymetrix PA0616_at
DNASU PaCD00006272
Entrez 877613
GenBank AAG04005.1
INSDC AAG04005.1
NCBI Locus Tag PA0616
protein_id(GenBank) gb|AAG04005.1|AE004497_12|gnl|PseudoCAP|PA0616
TIGR NTL03PA00617
UniParc UPI00000D4212
UniProtKB Acc G3XCT9
UniProtKB ID G3XCT9_PSEAE
UniRef100 UniRef100_W0YK78
UniRef50 UniRef50_Q1I683
UniRef90 UniRef90_W0YK78

Product

Feature Type CDS
Coding Frame 1
Product Name
R2 pyocin membrane-piercing spike
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.36
Kyte-Doolittle Hydrophobicity Value -0.012
Molecular Weight (kDa) 19.4
Isoelectric Point (pI) 6.39

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4S37 METAL BINDING PROTEIN 01/26/15 Crystal structure of R2 pyocin membrane-piercing spike Pseudomonas aeruginosa 2.2 X-RAY DIFFRACTION 100.0
4S36 METAL BINDING PROTEIN 01/26/15 Crystal structure of the C-terminal domain of R2 pyocin membrane-piercing spike Pseudomonas aeruginosa 1.46 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 63 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000599 (300 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0616

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Predicting drug targets by homology modelling of Pseudomonas aeruginosa proteins of unknown function.
Babic N, Kovacic F
PLoS One 2021;16(10):e0258385
PubMed ID: 34648550