Pseudomonas aeruginosa UCBPP-PA14, PA14_02310 (atsA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag
PA14_02310
Name
atsA
Replicon chromosome
Genomic location 206784 - 208394 (- strand)
Transposon Mutants 2 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq YP_788341.1
GI 116053904
Entrez 4383583
INSDC ABJ15140.1
NCBI Locus Tag PA14_02310
UniParc UPI000045D3A8
UniProtKB Acc Q02UP5
UniProtKB ID Q02UP5_PSEAB
UniRef100 UniRef100_Q02UP5
UniRef50 UniRef50_P51691
UniRef90 UniRef90_P51691

Product

Feature Type CDS
Coding Frame 1
Product Name
arylsulfatase
Evidence for Translation
Charge (pH 7) -12.15
Kyte-Doolittle Hydrophobicity Value -0.365
Molecular Weight (kDa) 59.8
Isoelectric Point (pI) 5.62

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4CYS HYDROLASE 04/14/14 G6 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa, in complex with Phenylphosphonic acid PSEUDOMONAS AERUGINOSA 1.88 X-RAY DIFFRACTION 97.8
4CYR HYDROLASE 04/14/14 G4 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa PSEUDOMONAS AERUGINOSA 1.72 X-RAY DIFFRACTION 97.8
4CXU HYDROLASE 04/08/14 G4 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa, in complex with 3-Br-Phenolphenylphosphonate PSEUDOMONAS AERUGINOSA 2.03 X-RAY DIFFRACTION 97.8
1HDH HYDROLASE 11/16/00 Arylsulfatase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 1.3 X-RAY DIFFRACTION 98.9
5AJ9 HYDROLASE 02/20/15 G7 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 97.6
4CXK HYDROLASE 04/07/14 G9 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa PSEUDOMONAS AERUGINOSA 1.86 X-RAY DIFFRACTION 97.2
4CXS HYDROLASE 04/08/14 G4 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa, in complex with Phenylphosphonic acid PSEUDOMONAS AERUGINOSA 2.3 X-RAY DIFFRACTION 97.8

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 241 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000181 (382 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_02310
Search term: atsA
Search term: arylsulfatase

Human Homologs

Ensembl 86, assembly GRCh38.p7
arylsulfatase B [Source:HGNC Symbol;Acc:HGNC:714]
E-value: 9.6e-28
Percent Identity: 34.7

Transposon Insertions in UCBPP-PA14

Type MAR2xT7 transposon (exoU background) insertion site
Mutant ID PA14NR: 6402
Genomic Position 207712
Position in ORF 929(1611)
Reference 16477005
Type MAR2xT7 transposon (wild type background) insertion site
Mutant ID PA14NR: 43813
Genomic Position 207329
Position in ORF 546(1611)
Reference 16477005

Transposon Insertions in Orthologs

Type ISlacZ/hah insertion site
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA0183
Mutant ID UWGC: 19684
Genomic Position 208954
Position in ORF 1032(1611)
Reference 14617778
Type Confirmed ISphoA/hah insertion site
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA0183
Mutant ID UWGC: 41442
Genomic Position 208707
Position in ORF 785(1611)
Reference 14617778

References

No references are associated with this feature.