Pseudomonas aeruginosa PAO1, PA1754 (cysB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1754
Name
cysB
Replicon chromosome
Genomic location 1894249 - 1895223 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250445.1
GI 15596951
Affymetrix PA1754_cysB_at
DNASU PaCD00006799
Entrez 883007
GenBank AAG05143.1
INSDC AAG05143.1
NCBI Locus Tag PA1754
protein_id(GenBank) gb|AAG05143.1|AE004601_8|gnl|PseudoCAP|PA1754
TIGR NTL03PA01755
UniParc UPI00000D4219
UniProtKB Acc G3XCU6
UniProtKB ID G3XCU6_PSEAE
UniRef100 UniRef100_W0YS31
UniRef50 UniRef50_P06614
UniRef90 UniRef90_Q4K8S3

Product

Feature Type CDS
Coding Frame 1
Product Name
transcriptional regulator CysB
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) 0.01
Kyte-Doolittle Hydrophobicity Value 0.019
Molecular Weight (kDa) 36.1
Isoelectric Point (pI) 7.00

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5Z4Z DNA BINDING PROTEIN 01/15/18 Crystal structure of PaCysB NTD domain with space group C2 Pseudomonas aeruginosa 2.053 X-RAY DIFFRACTION 100.0
5Z50 DNA BINDING PROTEIN 01/15/18 Crystal structure of PaCysB regulatory domain Pseudomonas aeruginosa 2.206 X-RAY DIFFRACTION 100.0
5Z4Y DNA BINDING PROTEIN 01/18/18 Crystal structure of PaCysB NTD domain with space group P4 Pseudomonas aeruginosa 2.401 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 535 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003639 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1754
Search term: cysB

Human Homologs

References

Cloning, sequence and mutagenesis of the structural gene of Pseudomonas aeruginosa CysB, which can activate algD transcription.
Delic-Attree I, Toussaint B, Garin J, Vignais PM
Mol Microbiol 1997 Jun;24(6):1275-84
PubMed ID: 9218775
Transcriptional control of the pvdS iron starvation sigma factor gene by the master regulator of sulfur metabolism CysB in Pseudomonas aeruginosa.
Imperi F, Tiburzi F, Fimia GM, Visca P
Environ Microbiol 2010 Jun;12(6):1630-42
PubMed ID: 20370820
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994