Pseudomonas aeruginosa PAO1, PA2018 (mexY)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2018
Name
mexY
Synonym: mexH
Synonym: amrB
Replicon chromosome
Genomic location 2208169 - 2211306 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 8 transposon mutants in orthologs

Cross-References

RefSeq NP_250708.1
GI 15597214
Affymetrix PA2018_at
Entrez 878882
GenBank AAG05406.1
INSDC AAG05406.1
NCBI Locus Tag PA2018
protein_id(GenBank) gb|AAG05406.1|AE004628_4|gnl|PseudoCAP|PA2018
TIGR NTL03PA02018
UniParc UPI00000D420B
UniProtKB Acc G3XCW2
UniProtKB ID G3XCW2_PSEAE
UniRef100 UniRef100_G3XCW2
UniRef50 UniRef50_P31224
UniRef90 UniRef90_G3XCW2

Product

Feature Type CDS
Coding Frame 1
Product Name
Resistance-Nodulation-Cell Division (RND) multidrug efflux transporter MexY
Product Name Confidence: Class 1
Synonyms RND multidrug efflux transporter
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.58
Kyte-Doolittle Hydrophobicity Value 0.341
Molecular Weight (kDa) 112.7
Isoelectric Point (pI) 6.24

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 2
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
mexY MexY is the RND-type membrane protein of the efflux complex MexXY-OprM. [PMID:9925549] ARO:3003033 protein homolog model Strict 1808.1 93.79 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 487 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000417 (2093 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2018
Search term: mexY

Human Homologs

References

Efflux-mediated aminoglycoside and macrolide resistance in Burkholderia pseudomallei.
Moore RA, DeShazer D, Reckseidler S, Weissman A, Woods DE
Antimicrob Agents Chemother 1999 Mar;43(3):465-70
PubMed ID: 10049252
MexXY multidrug efflux system of Pseudomonas aeruginosa.
Morita Y, Tomida J, Kawamura Y
Front Microbiol 2012;3:408
PubMed ID: 23233851
Involvement of an active efflux system in the natural resistance of Pseudomonas aeruginosa to aminoglycosides.
Aires JR, Köhler T, Nikaido H, Plésiat P
Antimicrob Agents Chemother 1999 Nov;43(11):2624-8
PubMed ID: 10543738
Contribution of the MexX-MexY-oprM efflux system to intrinsic resistance in Pseudomonas aeruginosa.
Masuda N, Sakagawa E, Ohya S, Gotoh N, Tsujimoto H, Nishino T
Antimicrob Agents Chemother 2000 Sep;44(9):2242-6
PubMed ID: 10952562
Induction of the MexXY efflux pump in Pseudomonas aeruginosa is dependent on drug-ribosome interaction.
Jeannot K, Sobel ML, El Garch F, Poole K, Plésiat P
J Bacteriol 2005 Aug;187(15):5341-6
PubMed ID: 16030228