Pseudomonas aeruginosa PAO1, PA3361 (lecB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3361
Name
lecB
Replicon chromosome
Genomic location 3773029 - 3773376 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
CommentBasolaterally applied LecB binds beta1-integrin and causes its internalization (PMID:32156827)
CommentLecB inhibits cell migration and epithelial wound healing (PMID:32156827)

Cross-References

RefSeq NP_252051.1
GI 15598557
Affymetrix PA3361_at
DNASU PaCD00007472
Entrez 882528
GenBank AAG06749.1
INSDC AAG06749.1
NCBI Locus Tag PA3361
protein_id(GenBank) gb|AAG06749.1|AE004758_6|gnl|PseudoCAP|PA3361
TIGR NTL03PA03361
UniParc UPI00000C59D3
UniProtKB Acc Q9HYN5
UniProtKB ID Q9HYN5_PSEAE
UniRef100 UniRef100_W0YU60
UniRef50 UniRef50_Q02QJ5
UniRef90 UniRef90_W0YU60

Product

Feature Type CDS
Coding Frame 1
Product Name
fucose-binding lectin PA-IIL
Product Name Confidence: Class 1
Synonyms LecB
Evidence for Translation
Charge (pH 7) -5.02
Kyte-Doolittle Hydrophobicity Value 0.054
Molecular Weight (kDa) 11.9
Isoelectric Point (pI) 3.83

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Outer Membrane Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1GZT LECTIN 06/06/02 Pseudomonas aeruginosa lectin II (PA-IIL) together with fucose PSEUDOMONAS AERUGINOSA 1.3 X-RAY DIFFRACTION 100.0
6S5R SUGAR BINDING PROTEIN 07/02/19 Cfucosylated second generation peptide dendrimer SBD6 bound to Fucose binding Lectin LecB (PA-IIL) from Pseudomonas aeruginosa at 2.08 Angstrom resolution, incomplete structure Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 2.076 X-RAY DIFFRACTION 100.0
6S5P SUGAR BINDING PROTEIN 07/02/19 Cfucosylated peptide SBL2 bound to Fucose binding Lectin LecB (PA-IIL) from Pseudomonas aeruginosa at 1.46 Angstrom resolution Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 1.46 X-RAY DIFFRACTION 100.0
7NEF ANTIBIOTIC 02/03/21 Fucosylated linear peptide Fln65 bound to the fucose binding lectin LecB PA-IIL from Pseudomonas aeruginosa at 1.5 Angstrom resolution Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 1.51 X-RAY DIFFRACTION 100.0
7NEW ANTIBIOTIC 02/05/21 Fucosylated heterochiral linear peptide Fdln69 bound to the fucose binding lectin LecB PA-IIL from Pseudomonas aeruginosa at 2.0 Angstrom resolution Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 2.02 X-RAY DIFFRACTION 100.0
3ZDV SUGAR BINDING PROTEIN 11/30/12 Crystal structure of the LecB lectin from Pseudomonas aeruginosa in complex with Methyl 6-(2,4,6-trimethylphenylsulfonylamido)-6-deoxy-alpha-D-mannopyranoside PSEUDOMONAS AERUGINOSA 1.41 X-RAY DIFFRACTION 100.0
2JDH SUGAR BINDING PROTEIN 01/09/07 Lectin PA-IIL of P.aeruginosa complexed with disaccharide derivative PSEUDOMONAS AERUGINOSA 1.1 X-RAY DIFFRACTION 100.0
2JDK LECTIN 01/10/07 Lectin PA-IIL of P.aeruginosa complexed with disaccharide derivative PSEUDOMONAS AERUGINOSA 1.1 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
Beta-D-Glucose DB02379 DrugBank 5.1.4 Q9HYN5 Fucose-binding lectin PA-IIL 100.0 115 3.3e-55
N-acetyl-alpha-D-glucosamine DB03740 DrugBank 5.1.4 Q9HYN5 Fucose-binding lectin PA-IIL 100.0 115 3.3e-55
Beta-D-Fructopyranose DB02561 DrugBank 5.1.4 Q9HYN5 Fucose-binding lectin PA-IIL 100.0 115 3.3e-55

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 7 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002267 (300 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3361
Search term: lecB

Human Homologs

References

Structural basis for oligosaccharide-mediated adhesion of Pseudomonas aeruginosa in the lungs of cystic fibrosis patients.
Mitchell E, Houles C, Sudakevitz D, Wimmerova M, Gautier C, Pérez S, Wu AM, Gilboa-Garber N, Imberty A
Nat Struct Biol 2002 Dec;9(12):918-21
PubMed ID: 12415289
Identification and characterization of pseudomonas aeruginosa PA-IIL lectin gene and protein compared to PA-IL.
Gilboa-Garber N, Katcoff DJ, Garber NC
FEMS Immunol Med Microbiol 2000 Sep;29(1):53-7
PubMed ID: 10967261
Pseudomonas aeruginosa lectin LecB is located in the outer membrane and is involved in biofilm formation.
Tielker D, Hacker S, Loris R, Strathmann M, Wingender J, Wilhelm S, Rosenau F, Jaeger KE
Microbiology (Reading) 2005 May;151(Pt 5):1313-1323
PubMed ID: 15870442
The Pseudomonas aeruginosa Lectin LecB Causes Integrin Internalization and Inhibits Epithelial Wound Healing.
Thuenauer R, Landi A, Trefzer A, Altmann S, Wehrum S, Eierhoff T, Diedrich B, Dengjel J, Nyström A, Imberty A, Römer W
mBio 2020 Mar 10;11(2)
PubMed ID: 32156827