Pseudomonas aeruginosa PAO1, PA3364 (amiC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3364
Name
amiC
Replicon chromosome
Genomic location 3774578 - 3775735 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252054.1
GI 15598560
Affymetrix PA3364_amiC_at
Entrez 877798
GenBank AAG06752.1
INSDC AAG06752.1
NCBI Locus Tag PA3364
protein_id(GenBank) gb|AAG06752.1|AE004758_9|gnl|PseudoCAP|PA3364
TIGR NTL03PA03364
UniParc UPI0000165ECF
UniProtKB Acc P27017
UniProtKB ID AMIC_PSEAE
UniRef100 UniRef100_P27017
UniRef50 UniRef50_P27017
UniRef90 UniRef90_P27017

Product

Feature Type CDS
Coding Frame 1
Product Name
aliphatic amidase expression-regulating protein
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.74
Kyte-Doolittle Hydrophobicity Value -0.313
Molecular Weight (kDa) 42.8
Isoelectric Point (pI) 5.82

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1PEA BINDING PROTEIN 11/16/95 AMIDE RECEPTOR/NEGATIVE REGULATOR OF THE AMIDASE OPERON OF PSEUDOMONAS AERUGINOSA (AMIC) COMPLEXED WITH ACETAMIDE Pseudomonas aeruginosa 2.1 X-RAY DIFFRACTION 98.7
1QNL TRANSFERASE 10/19/99 AMIDE RECEPTOR/NEGATIVE REGULATOR OF THE AMIDASE OPERON OF PSEUDOMONAS AERUGINOSA (AMIC) COMPLEXED WITH BUTYRAMIDE PSEUDOMONAS AERUGINOSA 2.7 X-RAY DIFFRACTION 98.4
1QO0 BINDING PROTEIN 10/26/99 Amide receptor of the amidase operon of Pseudomonas aeruginosa (AmiC) complexed with the negative regulator AmiR. PSEUDOMONAS AERUGINOSA 2.25 X-RAY DIFFRACTION 98.7

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
Butyramide DB02121 DrugBank 5.1.4 P27017 Aliphatic amidase expression-regulating protein 100.0 385 7.1e-227
Acetamide DB02736 DrugBank 5.1.4 P27017 Aliphatic amidase expression-regulating protein 100.0 385 7.1e-227

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 242 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002264 (348 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3364
Search term: amiC

Human Homologs

References

Antitermination of amidase expression in Pseudomonas aeruginosa is controlled by a novel cytoplasmic amide-binding protein.
Wilson SA, Wachira SJ, Drew RE, Jones D, Pearl LH
EMBO J 1993 Sep;12(9):3637-42
PubMed ID: 8253087
Cloning and DNA sequence of amiC, a new gene regulating expression of the Pseudomonas aeruginosa aliphatic amidase, and purification of the amiC product.
Wilson S, Drew R
J Bacteriol 1991 Aug;173(16):4914-21
PubMed ID: 1907262
Crystal structure of AmiC: the controller of transcription antitermination in the amidase operon of Pseudomonas aeruginosa.
Pearl L, O'Hara B, Drew R, Wilson S
EMBO J 1994 Dec 15;13(24):5810-7
PubMed ID: 7813419