Pseudomonas aeruginosa PAO1, PA3582 (glpK)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3582
Name
glpK
Replicon chromosome
Genomic location 4013685 - 4015202 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 5 transposon mutants in orthologs

Cross-References

RefSeq NP_252272.1
GI 15598778
Affymetrix PA3582_glpK_at
Entrez 880162
GenBank AAG06970.1
INSDC AAG06970.1
NCBI Locus Tag PA3582
protein_id(GenBank) gb|AAG06970.1|AE004779_2|gnl|PseudoCAP|PA3582
TIGR NTL03PA03582
UniParc UPI000012B791
UniProtKB Acc Q51390
UniProtKB ID GLPK2_PSEAE
UniRef100 UniRef100_Q51390
UniRef50 UniRef50_O66131
UniRef90 UniRef90_Q51390

Product

Feature Type CDS
Coding Frame 1
Product Name
glycerol kinase
Product Name Confidence: Class 1
Synonyms Glycerokinase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.78
Kyte-Doolittle Hydrophobicity Value -0.296
Molecular Weight (kDa) 56.0
Isoelectric Point (pI) 5.87

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 488 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002088 (832 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3582
Search term: glpK
Search term: glycerol kinase

Human Homologs

Ensembl 110, assembly GRCh38.p14
glycerol kinase [Source:HGNC Symbol;Acc:HGNC:4289]
E-value: 8.7e-129
Percent Identity: 49.4
Ensembl 110, assembly GRCh38.p14
glycerol kinase [Source:HGNC Symbol;Acc:HGNC:4289]
E-value: 8.7e-129
Percent Identity: 49.4

References

Structure and gene-polypeptide relationships of the region encoding glycerol diffusion facilitator (glpF) and glycerol kinase (glpK) of Pseudomonas aeruginosa.
Schweizer HP, Jump R, Po C
Microbiology (Reading) 1997 Apr;143 ( Pt 4):1287-1297
PubMed ID: 9141691