Pseudomonas aeruginosa PAO1, PA3644 (lpxA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3644
Name
lpxA
Replicon chromosome
Genomic location 4082185 - 4082961 (- strand)

Cross-References

RefSeq NP_252334.1
GI 15598840
Affymetrix PA3644_lpxA_at
Entrez 880493
GenBank AAG07032.1
INSDC AAG07032.1
NCBI Locus Tag PA3644
protein_id(GenBank) gb|AAG07032.1|AE004784_5|gnl|PseudoCAP|PA3644
TIGR NTL03PA03644
UniParc UPI000012E8B4
UniProtKB Acc Q9X6P4
UniProtKB ID LPXA_PSEAE
UniRef100 UniRef100_A6V1E4
UniRef50 UniRef50_P32201
UniRef90 UniRef90_A4XWS9

Product

Feature Type CDS
Coding Frame 1
Product Name
UDP-N-acetylglucosamine acyltransferase
Product Name Confidence: Class 2
Synonyms acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.30
Kyte-Doolittle Hydrophobicity Value -0.118
Molecular Weight (kDa) 28.0
Isoelectric Point (pI) 7.05

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7OKB TRANSFERASE 05/17/21 Crystal structure of Pseudomonas aeruginosa LpxA in complex with compound 45 Pseudomonas aeruginosa 3.58 X-RAY DIFFRACTION 100.0
7OK2 TRANSFERASE 05/17/21 Crystal structure of Pseudomonas aeruginosa LpxA in complex with compound 3 Pseudomonas aeruginosa (strain PA7) 2.89 X-RAY DIFFRACTION 100.0
7OKA TRANSFERASE 05/17/21 Crystal structure of Pseudomonas aeruginosa LpxA in complex with compound 14 Pseudomonas aeruginosa (strain PA7) 2.74 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 433 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002030 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3644
Search term: lpxA

Human Homologs

References

First committed step of lipid A biosynthesis in Escherichia coli: sequence of the lpxA gene.
Coleman J, Raetz CR
J Bacteriol 1988 Mar;170(3):1268-74
PubMed ID: 3277952
Structural characterization of the lipid A component of Pseudomonas aeruginosa wild-type and rough mutant lipopolysaccharides.
Kulshin VA, Zähringer U, Lindner B, Jäger KE, Dmitriev BA, Rietschel ET
Eur J Biochem 1991 Jun 15;198(3):697-704
PubMed ID: 1904818
A left-handed parallel beta helix in the structure of UDP-N-acetylglucosamine acyltransferase.
Raetz CR, Roderick SL
Science 1995 Nov 10;270(5238):997-1000
PubMed ID: 7481807