Pseudomonas aeruginosa PAO1, PA3654 (pyrH)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3654
Name
pyrH
Synonym: smbA
Replicon chromosome
Genomic location 4092231 - 4092968 (- strand)

Cross-References

RefSeq NP_252344.1
GI 15598850
Affymetrix PA3654_pyrH_at
DNASU PaCD00007601
Entrez 880536
GenBank AAG07042.1
INSDC AAG07042.1
NCBI Locus Tag PA3654
protein_id(GenBank) gb|AAG07042.1|AE004785_6|gnl|PseudoCAP|PA3654
TIGR NTL03PA03654
UniParc UPI0000132DB7
UniProtKB Acc O82852
UniProtKB ID PYRH_PSEAE
UniRef100 UniRef100_Q02RC6
UniRef50 UniRef50_Q4KHH4
UniRef90 UniRef90_Q4KHH4

Product

Feature Type CDS
Coding Frame 1
Product Name
uridylate kinase
Product Name Confidence: Class 2
Synonyms uridine monophosphate kinase
Evidence for Translation
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.37
Kyte-Doolittle Hydrophobicity Value 0.117
Molecular Weight (kDa) 26.3
Isoelectric Point (pI) 5.38

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 638 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002020 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3654
Search term: pyrH
Search term: uridylate kinase

Human Homologs

References

Identification and characterization of the smbA gene, a suppressor of the mukB null mutant of Escherichia coli.
Yamanaka K, Ogura T, Niki H, Hiraga S
J Bacteriol 1992 Dec;174(23):7517-26
PubMed ID: 1447125
Escherichia coli UMP-kinase, a member of the aspartokinase family, is a hexamer regulated by guanine nucleotides and UTP.
Serina L, Blondin C, Krin E, Sismeiro O, Danchin A, Sakamoto H, Gilles AM, Bârzu O
Biochemistry 1995 Apr 18;34(15):5066-74
PubMed ID: 7711027
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142