Pseudomonas aeruginosa PAO1, PA3732

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3732
Name
Synonym: yjfI
Replicon chromosome
Genomic location 4182785 - 4183405 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentTranslation confirmed using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer. Coordinates verified using RT-PCR (PMID:24291602).

Cross-References

RefSeq NP_252421
GI 15598927
Affymetrix PA3732_at
DNASU PaCD00007630
Entrez 880303
GenBank AAG07119.1
NCBI Locus Tag PA3732
protein_id(GenBank) gb|AAG07119.1|AE004792_4|gnl|PseudoCAP|PA3732
TIGR NTL03PA03732
UniProtKB Acc Q9HXR0
UniProtKB ID Q9HXR0_PSEAE

Product

Feature Type CDS
Coding Frame 1
Product Name
Uncharacterized protein
Product Name Confidence: Class 1
Synonyms conserved hypothetical protein
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -12.79
Kyte-Doolittle Hydrophobicity Value 0.214
Molecular Weight (kDa) 16.0
Isoelectric Point (pI) 3.97

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 27 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001951 (400 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3732

Human Homologs

References

Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes.
Burland V, Plunkett G 3rd, Sofia HJ, Daniels DL, Blattner FR
Nucleic Acids Res 1995 Jun 25;23(12):2105-19
PubMed ID: 7610040
LTQ-XL mass spectrometry proteome analysis expands the Pseudomonas aeruginosa AmpR regulon to include cyclic di-GMP phosphodiesterases and phosphoproteins, and identifies novel open reading frames.
Kumari H, Murugapiran SK, Balasubramanian D, Schneper L, Merighi M, Sarracino D, Lory S, Mathee K
J Proteomics 2014 Jan 16;96:328-342
PubMed ID: 24291602