Pseudomonas aeruginosa PAO1, PA3742 (rplS)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA3742
Name	rplS
Replicon	chromosome
Genomic location	4195008 - 4195358 (- strand)
Transposon Mutants in orthologs	1 transposon mutants in orthologs

Cross-References

RefSeq	NP_252431.1
GI	15598937
Affymetrix	PA3742_rplS_at
DNASU	PaCD00007633
Entrez	880339
GenBank	AAG07129.1
INSDC	AAG07129.1
NCBI Locus Tag	PA3742
protein_id(GenBank)	gb|AAG07129.1|AE004793_6|gnl|PseudoCAP|PA3742
TIGR	NTL03PA03742
UniParc	UPI0000133CB9
UniProtKB Acc	Q9HXQ2
UniProtKB ID	RL19_PSEAE
UniRef100	UniRef100_A6V126
UniRef50	UniRef50_Q87LT1
UniRef90	UniRef90_A4XXT4

Product

Feature Type	CDS
Coding Frame	1
Product Name	50S ribosomal protein L19 Product Name Confidence: Class 2
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	11.98
Kyte-Doolittle Hydrophobicity Value	-0.365
Molecular Weight (kDa)	13.0
Isoelectric Point (pI)	11.08

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
7UNV	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.7	ELECTRON MICROSCOPY	100.0
7UNW	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.6	ELECTRON MICROSCOPY	100.0
6SPB	RIBOSOME	09/01/19	Pseudomonas aeruginosa 50s ribosome from a clinical isolate with a mutation in uL6	Pseudomonas aeruginosa	2.82	ELECTRON MICROSCOPY	100.0
7UNU	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.9	ELECTRON MICROSCOPY	100.0
7UNR	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.9	ELECTRON MICROSCOPY	100.0
6SPD	RIBOSOME	09/01/19	Pseudomonas aeruginosa 50s ribosome from a clinical isolate	Pseudomonas aeruginosa	3.28	ELECTRON MICROSCOPY	100.0
6SPG	RIBOSOME	09/01/19	Pseudomonas aeruginosa 70s ribosome from a clinical isolate	Pseudomonas aeruginosa	3.34	ELECTRON MICROSCOPY	100.0
6SPF	RIBOSOME	09/01/19	Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate	Pseudomonas aeruginosa	2.89	ELECTRON MICROSCOPY	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 592 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001940 (537 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA3742 Search term: rplS Search term: 50S ribosomal protein L19

Human Homologs

References

Primary structure of protein L19 from the large subunit of Escherichia coli ribosomes. Brosius J, Arfsten U Biochemistry 1978 Feb 7;17(3):508-16 PubMed ID: 339951

The nucleotide sequence of an Escherichia coli operon containing genes for the tRNA(m1G)methyltransferase, the ribosomal proteins S16 and L19 and a 21-K polypeptide. Byström AS, Hjalmarsson KJ, Wikström PM, Björk GR EMBO J 1983;2(6):899-905 PubMed ID: 6357787

The localization of protein L19 on the surface of 50 S subunits of Escherichia coli aided by the use of mutants lacking protein L19. Stöffler G, Noah M, Stöffler-Meilicke M, Dabbs ER J Biol Chem 1984 Apr 10;259(7):4521-6 PubMed ID: 6423642