Pseudomonas aeruginosa PAO1, PA3743 (trmD)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA3743
Name	trmD
Replicon	chromosome
Genomic location	4195400 - 4196158 (- strand)
Transposon Mutants in orthologs	1 transposon mutants in orthologs

Cross-References

RefSeq	NP_252432.1
GI	15598938
Affymetrix	PA3743_trmD_at
Entrez	880357
GenBank	AAG07130.1
INSDC	AAG07130.1
NCBI Locus Tag	PA3743
protein_id(GenBank)	gb|AAG07130.1|AE004793_7|gnl|PseudoCAP|PA3743
TIGR	NTL03PA03743
UniParc	UPI0000137422
UniProtKB Acc	Q9HXQ1
UniProtKB ID	TRMD_PSEAE
UniRef100	UniRef100_A6V125
UniRef50	UniRef50_B2JF31
UniRef90	UniRef90_A6V125

Product

Feature Type	CDS
Coding Frame	1
Product Name	tRNA (guanine-N1)-methyltransferase Product Name Confidence: Class 2
Synonyms	TrmD
Evidence for Translation
Charge (pH 7)	-9.61
Kyte-Doolittle Hydrophobicity Value	-0.461
Molecular Weight (kDa)	28.4
Isoelectric Point (pI)	4.89

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543 Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
6JOE	TRANSFERASE	03/20/19	Crystal structure of TrmD from Pseudomonas aeruginosa in complex with active-site inhibitor	Pseudomonas aeruginosa UCBPP-PA14	2.21	X-RAY DIFFRACTION	99.6
5ZHN	TRANSFERASE	03/13/18	Crystal structure of TrmD from Pseudomonas aeruginosa in complex with active-site inhibitor	Pseudomonas aeruginosa UCBPP-PA14	2.65	X-RAY DIFFRACTION	99.6
6AFK	TRANSFERASE	08/08/18	Crystal structure of TrmD from Pseudomonas aeruginosa in complex with active-site inhibitor	Pseudomonas aeruginosa UCBPP-PA14	2.75	X-RAY DIFFRACTION	99.6
5ZHM	TRANSFERASE	03/13/18	Crystal structure of TrmD from Pseudomonas aeruginosa in complex with active-site inhibitor	Pseudomonas aeruginosa	2.76	X-RAY DIFFRACTION	99.6
6JKI	TRANSFERASE	03/01/19	Crystal structure and catalytic mechanism of the essential m1G37 tRNA methyltransferase TrmD from Pseudomonas aeruginosa	Pseudomonas aeruginosa	2.59	X-RAY DIFFRACTION	99.6
5WYQ	TRANSFERASE	01/15/17	Crystal Structure and catalytic mechanism of the essential m1G37 tRNA methyltransferase TrmD from Pseudomonas aeruginosa	Pseudomonas aeruginosa (strain UCBPP-PA14)	2.16	X-RAY DIFFRACTION	99.6
5WYR	TRANSFERASE	01/15/17	Crystal structure and catalytic mechanism of the essential m1G37 tRNA methyltransferase TrmD from Pseudomonas aeruginosa	Pseudomonas aeruginosa (strain UCBPP-PA14)	2.45	X-RAY DIFFRACTION	99.6

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 591 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001939 (537 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA3743 Search term: trmD Search term: tRNA (guanine-N1)-methyltransferase

Human Homologs

References

Purification and characterization of transfer RNA (guanine-1)methyltransferase from Escherichia coli. Hjalmarsson KJ, Byström AS, Björk GR J Biol Chem 1983 Jan 25;258(2):1343-51 PubMed ID: 6337136

The nucleotide sequence of an Escherichia coli operon containing genes for the tRNA(m1G)methyltransferase, the ribosomal proteins S16 and L19 and a 21-K polypeptide. Byström AS, Hjalmarsson KJ, Wikström PM, Björk GR EMBO J 1983;2(6):899-905 PubMed ID: 6357787