Pseudomonas aeruginosa PAO1, PA3763 (purL)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3763
Name
purL
Replicon chromosome
Genomic location 4215544 - 4219440 (- strand)
Transposon Mutants 7 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252452.1
GI 15598958
Affymetrix PA3763_purL_at
Entrez 879752
GenBank AAG07150.1
INSDC AAG07150.1
NCBI Locus Tag PA3763
protein_id(GenBank) gb|AAG07150.1|AE004795_2|gnl|PseudoCAP|PA3763
TIGR NTL03PA03763
UniParc UPI0000132A5B
UniProtKB Acc Q9HXN2
UniProtKB ID PUR4_PSEAE
UniRef100 UniRef100_Q9HXN2
UniRef50 UniRef50_P15254
UniRef90 UniRef90_Q9HXN2

Product

Feature Type CDS
Coding Frame 1
Product Name
phosphoribosylformylglycinamidine synthase
Product Name Confidence: Class 2
Synonyms formylglycinamide ribotide amidotransferase
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -47.15
Kyte-Doolittle Hydrophobicity Value -0.165
Molecular Weight (kDa) 140.6
Isoelectric Point (pI) 4.81

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database Victors : 4233
Category
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 14641575

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 621 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001918 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3763
Search term: purL

Human Homologs

Ensembl 110, assembly GRCh38.p14
phosphoribosylformylglycinamidine synthase [Source:HGNC Symbol;Acc:HGNC:8863]
E-value: 9.8e-225
Percent Identity: 36.7

References

The organization of the purL gene encoding 5'-phosphoribosylformylglycinamide amidotransferase of Escherichia coli.
Sampei G, Mizobuchi K
J Biol Chem 1989 Dec 15;264(35):21230-8
PubMed ID: 2531746
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994