Pseudomonas aeruginosa PAO1, PA5489 (dsbA)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA5489
Name	dsbA
Replicon	chromosome
Genomic location	6180942 - 6181577 (- strand)
Transposon Mutants	1 transposon mutants in PAO1
Transposon Mutants in orthologs	2 transposon mutants in orthologs

Cross-References

RefSeq	NP_254176.1
GI	15600682
Affymetrix	PA5489_dsbA_at
DNASU	PaCD00008364
Entrez	877731
GenBank	AAG08874.1
INSDC	AAG08874.1
NCBI Locus Tag	PA5489
protein_id(GenBank)	gb|AAG08874.1|AE004961_9|gnl|PseudoCAP|PA5489
TIGR	NTL03PA05490
UniParc	UPI00001298C0
UniProtKB Acc	P0C2B2
UniProtKB ID	DSBA_PSEAE
UniRef100	UniRef100_Q02DM0
UniRef50	UniRef50_O52376
UniRef90	UniRef90_Q02DM0

Product

Feature Type	CDS
Coding Frame	1
Product Name	thiol:disulfide interchange protein DsbA Product Name Confidence: Class 2
Synonyms
Evidence for Translation	Detected by MALDI-TOF/TOF (PMID:19333994). Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-2.38
Kyte-Doolittle Hydrophobicity Value	-0.064
Molecular Weight (kDa)	23.4
Isoelectric Point (pI)	6.40

Subcellular localization

Individual Mappings	Localization Confidence PMID Periplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
4ZL9	OXIDOREDUCTASE	05/01/15	Crystal structure of Pseudomonas aeruginosa DsbA E82I: Crystal III	Pseudomonas aeruginosa	1.7	X-RAY DIFFRACTION	98.4
4ZL7	OXIDOREDUCTASE	05/01/15	Crystal structure of Pseudomonas aeruginosa DsbA E82I: Crystal I	Pseudomonas aeruginosa	1.922	X-RAY DIFFRACTION	98.4
5TLQ	OXIDOREDUCTASE	10/11/16	Model structure of the oxidized PaDsbA1 and 3-[(2-methylbenzyl)sulfanyl]-4H-1,2,4-triazol-4-amine complex	Pseudomonas aeruginosa	NOT	SOLUTION NMR	100.0
5DCH	OXIDOREDUCTASE	08/24/15	Crystal structure of Pseudomonas aeruginosa DsbA E82I in complex with MIPS-0000851 (3-[(2-METHYLBENZYL)SULFANYL]-4H-1,2,4-TRIAZOL-4-AMINE)	Pseudomonas aeruginosa	1.447	X-RAY DIFFRACTION	98.4
3H93	TRANSCRIPTION REGULATOR	04/29/09	Crystal Structure of Pseudomonas aeruginosa DsbA	Pseudomonas aeruginosa PAO1	1.501	X-RAY DIFFRACTION	99.0
4ZL8	OXIDOREDUCTASE	05/01/15	Crystal structure of Pseudomonas aeruginosa DsbA E82I: Crystal II	Pseudomonas aeruginosa	1.395	X-RAY DIFFRACTION	98.4
2MBT	OXIDOREDUCTASE	08/03/13	NMR study of PaDsbA	Pseudomonas aeruginosa	NOT	SOLUTION NMR	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 149 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG005029 (534 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA5489 Search term: dsbA Search term: thiol:disulfide interchange protein DsbA

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. Lewenza S, Gardy JL, Brinkman FS, Hancock RE Genome Res 2005 Feb;15(2):321-9 PubMed ID: 15687295

Cloning and expression of the gene for a protein disulfide oxidoreductase from Azotobacter vinelandii: complementation of an Escherichia coli dsbA mutant strain. Ng TC, Kwik JF, Maier RJ Gene 1997 Mar 25;188(1):109-13 PubMed ID: 9099867

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen. Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM Proteomics 2009 Apr;9(7):1901-15 PubMed ID: 19333994