Pseudomonas aeruginosa PAO1, PA0071 (tagR1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0071
Name
tagR1
Replicon chromosome
Genomic location 83380 - 85092 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248761.1
GI 15595269
Affymetrix PA0071_at
Entrez 879195
GenBank AAG03461.1
INSDC AAG03461.1
NCBI Locus Tag PA0071
protein_id(GenBank) gb|AAG03461.1|AE004446_9|gnl|PseudoCAP|PA0071
TIGR NTL03PA00072
UniParc UPI00000C4F18
UniProtKB Acc Q9I761
UniProtKB ID Q9I761_PSEAE
UniRef100 UniRef100_Q9I761
UniRef50 UniRef50_Q4K3Q0
UniRef90 UniRef90_Q9I761

Product

Feature Type CDS
Coding Frame 1
Product Name
TagR1
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -3.86
Kyte-Doolittle Hydrophobicity Value -0.303
Molecular Weight (kDa) 61.9
Isoelectric Point (pI) 5.87

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
Unknown Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database VFDB : VF0334
Category Hcp secretion island-1 encoded type VI secretion system (H-T6SS)
Evidence ECO:0000305
curator inference used in manual assertion
Host Organism
Notes
Reference 16763151

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 91 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000070 (433 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0071
Search term: tagR1
Search term: TagR1

References

A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD
Cell Host Microbe 2010 Jan;7(1):25-37
PubMed ID: 20114026
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology (Reading, Engl.) 2007 Aug;153(Pt 8):2689-99
PubMed ID: 17660433
TagR promotes PpkA-catalysed type VI secretion activation in Pseudomonas aeruginosa.
Hsu F, Schwarz S, Mougous JD
Mol. Microbiol. 2009 Jun;72(5):1111-25
PubMed ID: 19400797
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994