Pseudomonas aeruginosa PAO1, PA0078 (tssL1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0078
Name
tssL1
Replicon chromosome
Genomic location 95048 - 96397 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_248768.1
GI 15595276
Affymetrix PA0078_at
Entrez 879392
GenBank AAG03468.1
INSDC AAG03468.1
NCBI Locus Tag PA0078
protein_id(GenBank) gb|AAG03468.1|AE004447_1|gnl|PseudoCAP|PA0078
TIGR NTL03PA00079
UniParc UPI00000C4F1F
UniProtKB Acc Q9I754
UniProtKB ID Q9I754_PSEAE
UniRef100 UniRef100_Q9I754
UniRef50 UniRef50_Q4K3P3
UniRef90 UniRef90_Q9I754

Product

Feature Type CDS
Coding Frame 1
Product Name
TssL1
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 3.33
Kyte-Doolittle Hydrophobicity Value -0.256
Molecular Weight (kDa) 49.0
Isoelectric Point (pI) 8.12

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4B62 MEMBRANE PROTEIN 08/08/12 The structure of the cell wall anchor of the T6SS from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA PAO1 1.45 X-RAY DIFFRACTION 99.3

Virulence Evidence

Source 1
Database VFDB : VF0334
Category Hcp secretion island-1 encoded type VI secretion system (H-T6SS)
Evidence ECO:0000305
curator inference used in manual assertion
Host Organism
Notes
Reference 16763151

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 392 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000077 (855 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0078
Search term: tssL1
Search term: TssL1

References

A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD
Cell Host Microbe 2010 Jan;7(1):25-37
PubMed ID: 20114026
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology (Reading, Engl.) 2007 Aug;153(Pt 8):2689-99
PubMed ID: 17660433