Pseudomonas aeruginosa PAO1, PA0172 (siaA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0172
Name
siaA
Replicon chromosome
Genomic location 194757 - 196748 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_248862.1
GI 15595370
Affymetrix PA0172_at
Entrez 879545
GenBank AAG03562.1
INSDC AAG03562.1
NCBI Locus Tag PA0172
protein_id(GenBank) gb|AAG03562.1|AE004455_3|gnl|PseudoCAP|PA0172
TIGR NTL03PA00173
UniParc UPI00000C4F7B
UniProtKB Acc Q9I6W0
UniProtKB ID Q9I6W0_PSEAE
UniRef100 UniRef100_Q9I6W0
UniRef50 UniRef50_Q9I6W0
UniRef90 UniRef90_Q9I6W0

Product

Feature Type CDS
Coding Frame 1
Product Name
SiaA
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.03
Kyte-Doolittle Hydrophobicity Value -0.206
Molecular Weight (kDa) 74.0
Isoelectric Point (pI) 7.00

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown (This protein may have multiple localization sites) Class 3
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 243 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000170 (331 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0172
Search term: siaA
Search term: SiaA

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 42259
Genomic Position 196392
Position in ORF 1636(1992)
Reference 14617778
Type ISlacZ/hah insertion site
Mutant ID UWGC: 15803
Genomic Position 195510
Position in ORF 754(1992)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (exoU background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_02150
Mutant ID PA14NR: 5762
Genomic Position 195250
Position in ORF 1634(1992)
Reference 16477005
Type PhoA transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_02150
Mutant ID PA14NR: 476
Genomic Position 194427
Position in ORF 811(1992)
Reference 16477005

References

Detergent-induced cell aggregation in subpopulations of Pseudomonas aeruginosa as a preadaptive survival strategy.
Klebensberger J, Lautenschlager K, Bressler D, Wingender J, Philipp B.
Environ Microbiol. 2007 Sep;9(9):2247-59.
PubMed ID: 17686022
SiaA and SiaD are essential for inducing autoaggregation as a specific response to detergent stress in Pseudomonas aeruginosa.
Klebensberger J, Birkenmaier A, Geffers R, Kjelleberg S, Philipp B.
Environ Microbiol. 2009 Dec;11(12):3073-86.
PubMed ID: 19638175
SiaA/D Interconnects c-di-GMP and RsmA Signaling to Coordinate Cellular Aggregation of Pseudomonas aeruginosa in Response to Environmental Conditions.
Colley B(1), Dederer V(2), Carnell M(3), Kjelleberg S(4), Rice SA(4), Klebensberger J(2).
Front Microbiol. 2016 Feb 26;7:179.
PubMed ID: 26955366