Pseudomonas aeruginosa PAO1, PA0172 (siaA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0172
Name
siaA
Replicon chromosome
Genomic location 194757 - 196748 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_248862.1
GI 15595370
Affymetrix PA0172_at
Entrez 879545
GenBank AAG03562.1
INSDC AAG03562.1
NCBI Locus Tag PA0172
protein_id(GenBank) gb|AAG03562.1|AE004455_3|gnl|PseudoCAP|PA0172
TIGR NTL03PA00173
UniParc UPI00000C4F7B
UniProtKB Acc Q9I6W0
UniProtKB ID Q9I6W0_PSEAE
UniRef100 UniRef100_Q9I6W0
UniRef50 UniRef50_Q9I6W0
UniRef90 UniRef90_Q9I6W0

Product

Feature Type CDS
Coding Frame 1
Product Name
SiaA
Product Name Confidence: Class 1
CommentInvolved in surfactant-induced (SDS) aggregation.
CommentInvolved in postranscriptional regulation of CupA fimbriae via transcriptional activation of rsmZ
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.03
Kyte-Doolittle Hydrophobicity Value -0.206
Molecular Weight (kDa) 74.0
Isoelectric Point (pI) 7.00

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown (This protein may have multiple localization sites) Class 3
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 243 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000170 (328 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0172
Search term: siaA
Search term: SiaA

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 42259
Genomic Position 196392
Position in ORF 1636(1992)
Reference 14617778
Type ISlacZ/hah insertion site
Mutant ID UWGC: 15803
Genomic Position 195510
Position in ORF 754(1992)
Reference 14617778

Transposon Insertions in Orthologs

References

Detergent-induced cell aggregation in subpopulations of Pseudomonas aeruginosa as a preadaptive survival strategy.
Klebensberger J, Lautenschlager K, Bressler D, Wingender J, Philipp B
Environ. Microbiol. 2007 Sep;9(9):2247-59
PubMed ID: 17686022
SiaA and SiaD are essential for inducing autoaggregation as a specific response to detergent stress in Pseudomonas aeruginosa.
Klebensberger J, Birkenmaier A, Geffers R, Kjelleberg S, Philipp B
Environ. Microbiol. 2009 Dec;11(12):3073-86
PubMed ID: 19638175
SiaA/D Interconnects c-di-GMP and RsmA Signaling to Coordinate Cellular Aggregation of Pseudomonas aeruginosa in Response to Environmental Conditions.
Colley B, Dederer V, Carnell M, Kjelleberg S, Rice SA, Klebensberger J
Front Microbiol 2016 ;7
PubMed ID: 26955366