Pseudomonas aeruginosa PAO1, PA0196 (pntB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0196
Name
pntB
Replicon chromosome
Genomic location 225603 - 227039 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248887.1
GI 15595393
Affymetrix PA0196_pntB_at
Entrez 878146
GenBank AAG03585.1
INSDC AAG03585.1
NCBI Locus Tag PA0196
protein_id(GenBank) gb|AAG03585.1|AE004457_6|gnl|PseudoCAP|PA0196
TIGR NTL03PA00197
UniParc UPI00000C4F8F
UniProtKB Acc Q9I6T9
UniProtKB ID Q9I6T9_PSEAE
UniRef100 UniRef100_W0YJM3
UniRef50 UniRef50_Q92JG3
UniRef90 UniRef90_Q87U51

Product

Feature Type CDS
Coding Frame 1
Product Name
pyridine nucleotide transhydrogenase, beta subunit
Product Name Confidence: Class 2
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.86
Kyte-Doolittle Hydrophobicity Value 0.646
Molecular Weight (kDa) 49.9
Isoelectric Point (pI) 7.27

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 2
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 305 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000195 (557 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0196
Search term: pntB

Human Homologs

Ensembl 86, assembly GRCh38.p7
nicotinamide nucleotide transhydrogenase [Source:HGNC Symbol;Acc:HGNC:7863]
E-value: 1.9e-112
Percent Identity: 47.7

References

Nucleotide sequence of the pntA and pntB genes encoding the pyridine nucleotide transhydrogenase of Escherichia coli.
Clarke DM, Loo TW, Gillam S, Bragg PD
Eur. J. Biochem. 1986 Aug;158(3):647-53
PubMed ID: 3525165
A mutation at Gly314 of the beta subunit of the Escherichia coli pyridine nucleotide transhydrogenase abolishes activity and affects the NADP(H)-induced conformational change.
Ahmad S, Glavas NA, Bragg PD
Eur. J. Biochem. 1992 Jul;207(2):733-9
PubMed ID: 1633824