Pseudomonas aeruginosa PAO1, PA0196 (pntB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0196
Name
pntB
Replicon chromosome
Genomic location 225603 - 227039 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_248887.1
GI 15595393
Affymetrix PA0196_pntB_at
Entrez 878146
GenBank AAG03585.1
INSDC AAG03585.1
NCBI Locus Tag PA0196
protein_id(GenBank) gb|AAG03585.1|AE004457_6|gnl|PseudoCAP|PA0196
TIGR NTL03PA00197
UniParc UPI00000C4F8F
UniProtKB Acc Q9I6T9
UniProtKB ID Q9I6T9_PSEAE
UniRef100 UniRef100_W0YJM3
UniRef50 UniRef50_Q92JG3
UniRef90 UniRef90_Q87U51

Product

Feature Type CDS
Coding Frame 1
Product Name
pyridine nucleotide transhydrogenase, beta subunit
Product Name Confidence: Class 2
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.86
Kyte-Doolittle Hydrophobicity Value 0.646
Molecular Weight (kDa) 49.9
Isoelectric Point (pI) 7.27

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 2
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 305 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000195 (557 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0196
Search term: pntB

Human Homologs

Ensembl 86, assembly GRCh38.p7
nicotinamide nucleotide transhydrogenase [Source:HGNC Symbol;Acc:HGNC:7863]
E-value: 1.9e-112
Percent Identity: 47.7

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 33088
Genomic Position 226179
Position in ORF 577(1437)
Reference 14617778
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 51459
Genomic Position 226974
Position in ORF 1372(1437)
Reference 14617778

Transposon Insertions in Orthologs

References

Nucleotide sequence of the pntA and pntB genes encoding the pyridine nucleotide transhydrogenase of Escherichia coli.
Clarke DM, Loo TW, Gillam S, Bragg PD
Eur. J. Biochem. 1986 Aug;158(3):647-53
PubMed ID: 3525165
A mutation at Gly314 of the beta subunit of the Escherichia coli pyridine nucleotide transhydrogenase abolishes activity and affects the NADP(H)-induced conformational change.
Ahmad S, Glavas NA, Bragg PD
Eur. J. Biochem. 1992 Jul;207(2):733-9
PubMed ID: 1633824