Pseudomonas aeruginosa PAO1, PA0265 (davD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0265
Name
davD
Synonym: gabD
Replicon chromosome
Genomic location 299522 - 300973 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1

Cross-References

RefSeq NP_248956.1
GI 15595462
Affymetrix PA0265_gabD_at
Entrez 881913
GenBank AAG03654.1
INSDC AAG03654.1
NCBI Locus Tag PA0265
protein_id(GenBank) gb|AAG03654.1|AE004464_7|gnl|PseudoCAP|PA0265
TIGR NTL03PA00266
UniParc UPI00000C4FCD
UniProtKB Acc Q9I6M5
UniProtKB ID DAVD_PSEAE
UniRef100 UniRef100_Q9I6M5
UniRef50 UniRef50_P25526
UniRef90 UniRef90_Q9I6M5

Product

Feature Type CDS
Coding Frame 1
Product Name
glutaric semialdehyde dehydrogenase
Product Name Confidence: Class 2
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected by iTRAQ (PMID:21863142).
Charge (pH 7) -4.41
Kyte-Doolittle Hydrophobicity Value -0.031
Molecular Weight (kDa) 51.6
Isoelectric Point (pI) 5.43

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 604 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000262 (559 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0265
Search term: davD

Human Homologs

Ensembl 86, assembly GRCh38.p7
aldehyde dehydrogenase 5 family member A1 [Source:HGNC Symbol;Acc:HGNC:408]
E-value: 1.8e-158
Percent Identity: 57.9

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 18230
Genomic Position 300265
Position in ORF 744(1452)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 32654
Genomic Position 300287
Position in ORF 766(1452)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_67_H5
Genomic Position 300418
Position in ORF 897(1452)
Reference 15805499

Transposon Insertions in Orthologs

References

Isolation and properties of Escherichia coli K-12 mutants impaired in the utilization of gamma-aminobutyrate.
Metzer E, Levitz R, Halpern YS
J. Bacteriol. 1979 Mar;137(3):1111-8
PubMed ID: 374339
Molecular organization of the Escherichia coli gab cluster: nucleotide sequence of the structural genes gabD and gabP and expression of the GABA permease gene.
Niegemann E, Schulz A, Bartsch K
Arch. Microbiol. 1993 ;160(6):454-60
PubMed ID: 8297211
Functional characterization of seven γ-Glutamylpolyamine synthetase genes and the bauRABCD locus for polyamine and β-Alanine utilization in Pseudomonas aeruginosa PAO1.
Yao X, He W, Lu CD
J. Bacteriol. 2011 Aug;193(15):3923-30
PubMed ID: 21622750
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011 ;2
PubMed ID: 21863142
Multiple and interconnected pathways for L-lysine catabolism in Pseudomonas putida KT2440.
Revelles O, Espinosa-Urgel M, Fuhrer T, Sauer U, Ramos JL
J. Bacteriol. 2005 Nov;187(21):7500-10
PubMed ID: 16237033