Pseudomonas aeruginosa PAO1, PA0266 (davT)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0266
Name
davT
Synonym: gabT
Replicon chromosome
Genomic location 301218 - 302498 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_248957.1
GI 15595463
Affymetrix PA0266_gabT_at
Entrez 880917
GenBank AAG03655.1
INSDC AAG03655.1
NCBI Locus Tag PA0266
protein_id(GenBank) gb|AAG03655.1|AE004465_1|gnl|PseudoCAP|PA0266
TIGR NTL03PA00267
UniParc UPI00000C4FCE
UniProtKB Acc Q9I6M4
UniProtKB ID DAVT_PSEAE
UniRef100 UniRef100_Q9I6M4
UniRef50 UniRef50_P50457
UniRef90 UniRef90_Q9I6M4

Product

Feature Type CDS
Coding Frame 1
Product Name
delta-aminovalerate aminotransferase
Product Name Confidence: Class 2
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -3.00
Kyte-Doolittle Hydrophobicity Value 0.142
Molecular Weight (kDa) 45.2
Isoelectric Point (pI) 6.43

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 639 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000263 (570 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0266
Search term: davT

Human Homologs

Ensembl 86, assembly GRCh38.p7
ornithine aminotransferase [Source:HGNC Symbol;Acc:HGNC:8091]
E-value: 2.3e-48
Percent Identity: 32.1

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 2478
Genomic Position 302250
Position in ORF 1033(1281)
Reference 14617778
Type ISlacZ/hah insertion site
Mutant ID UWGC: 15981
Genomic Position 301616
Position in ORF 399(1281)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Non-transcriptional fusion)
Mutant ID UBC: PAO1_lux_97_B4
Genomic Position 301887
Position in ORF 670(1281)
Reference 15805499

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_03450
Mutant ID PA14NR: 40751
Genomic Position 315489
Position in ORF 131(1281)
Reference 16477005
Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_03450
Mutant ID PA14NR: 53316
Genomic Position 316541
Position in ORF 1183(1281)
Reference 16477005
Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_03450
Mutant ID PA14NR: 27864
Genomic Position 315824
Position in ORF 466(1281)
Reference 16477005

References

Stereospecific production of the herbicide phosphinothricin (glufosinate) by transamination: isolation and characterization of a phosphinothricin-specific transaminase from Escherichia coli.
Schulz A, Taggeselle P, Tripier D, Bartsch K.
Appl Environ Microbiol. 1990 Jan;56(1):1-6.
PubMed ID: 2178550
Molecular analysis of two genes of the Escherichia coli gab cluster: nucleotide sequence of the glutamate:succinic semialdehyde transaminase gene (gabT) and characterization of the succinic semialdehyde dehydrogenase gene (gabD).
Bartsch K, von Johnn-Marteville A, Schulz A.
J Bacteriol. 1990 Dec;172(12):7035-42.
PubMed ID: 2254272
Functional Characterization of Seven {gamma}-Glutamylpolyamine Synthetase Genes and the bauRABCD Locus for Polyamine and {beta}-Alanine Utilization in Pseudomonas aeruginosa PAO1.
Yao X, He W, Lu CD.
J Bacteriol. 2011 Aug;193(15):3923-30.
PubMed ID: 21622750
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM.
Proteomics. 2009 Apr;9(7):1901-15.
PubMed ID: 19333994
Multiple and interconnected pathways for L-lysine catabolism in Pseudomonas putida KT2440.
Revelles O(1), Espinosa-Urgel M, Fuhrer T, Sauer U, Ramos JL.
J Bacteriol. 2005 Nov;187(21):7500-10.
PubMed ID: 16237033