Pseudomonas aeruginosa PAO1, PA0321

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0321
Name
Replicon chromosome
Genomic location 360413 - 361447 (- strand)
Transposon Mutants 3 transposon mutants in PAO1

Cross-References

RefSeq NP_249012.1
GI 15595518
Affymetrix PA0321_at
DNASU PaCD00006140
Entrez 878360
GenBank AAG03710.1
INSDC AAG03710.1
NCBI Locus Tag PA0321
protein_id(GenBank) gb|AAG03710.1|AE004470_4|gnl|PseudoCAP|PA0321
TIGR NTL03PA00322
UniParc UPI00000C5004
UniProtKB Acc Q9I6H0
UniProtKB ID Q9I6H0_PSEAE
UniRef100 UniRef100_A0A0C7ATC8
UniRef50 UniRef50_A6UYC4
UniRef90 UniRef90_A6UYC4

Product

Feature Type CDS
Coding Frame 1
Product Name
acetylpolyamine amidohydrolase
Product Name Confidence: Class 1
CommentPA0321 has been demonsrated to remove the acetyl group from the shorter acetyl-putrescine and acetyl-cadaverine
Evidence for Translation
Charge (pH 7) -8.21
Kyte-Doolittle Hydrophobicity Value -0.211
Molecular Weight (kDa) 37.9
Isoelectric Point (pI) 5.72

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 439 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000318 (315 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0321

Transposon Insertions in PAO1

Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 36636
Genomic Position 360538
Position in ORF 126(1035)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Non-transcriptional fusion)
Mutant ID UBC: PAO1_lux_50_C5
Genomic Position 360586
Position in ORF 174(1035)
Reference 15805499
Type mini-Tn5 lux transposon insertion site (Non-transcriptional fusion)
Mutant ID UBC: PAO1_lux_41_D3
Genomic Position 360597
Position in ORF 185(1035)
Reference 15805499

Transposon Insertions in Orthologs

References

Crystallization and some properties of acetylpolyamine amidohydrolase from Mycoplana bullata.
Fujishiro K, Ando M, Uwajima T
Biochem. Biophys. Res. Commun. 1988 Dec;157(3):1169-74
PubMed ID: 3207420
Acetylpolyamine amidohydrolase from Mycoplana ramosa: gene cloning and characterization of the metal-substituted enzyme.
Sakurada K, Ohta T, Fujishiro K, Hasegawa M, Aisaka K
J. Bacteriol. 1996 Oct;178(19):5781-6
PubMed ID: 8824626
Transcriptome analysis of agmatine and putrescine catabolism in Pseudomonas aeruginosa PAO1.
Chou HT, Kwon DH, Hegazy M, Lu CD
J. Bacteriol. 2008 Mar;190(6):1966-75
PubMed ID: 18192388
A fluorescence lifetime-based binding assay for acetylpolyamine amidohydrolases from Pseudomonas aeruginosa using a [1,3]dioxolo[4,5-f][1,3]benzodioxole (DBD) ligand probe.
Meyners C, Wawrzinek R, Krämer A, Hinz S, Wessig P, Meyer-Almes FJ
Anal Bioanal Chem 2014 Aug;406(20):4889-97
PubMed ID: 24871864
Substrate specificity and function of acetylpolyamine amidohydrolases from Pseudomonas aeruginosa.
Krämer A, Herzer J, Overhage J, Meyer-Almes FJ
BMC Biochem. 2016 Mar;17
PubMed ID: 26956223