Pseudomonas aeruginosa PAO1, PA0321

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0321
Name
Replicon chromosome
Genomic location 360413 - 361447 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249012.1
GI 15595518
Affymetrix PA0321_at
DNASU PaCD00006140
Entrez 878360
GenBank AAG03710.1
INSDC AAG03710.1
NCBI Locus Tag PA0321
protein_id(GenBank) gb|AAG03710.1|AE004470_4|gnl|PseudoCAP|PA0321
TIGR NTL03PA00322
UniParc UPI00000C5004
UniProtKB Acc Q9I6H0
UniProtKB ID Q9I6H0_PSEAE
UniRef100 UniRef100_A0A0C7ATC8
UniRef50 UniRef50_A6UYC4
UniRef90 UniRef90_A6UYC4

Product

Feature Type CDS
Coding Frame 1
Product Name
acetylpolyamine amidohydrolase
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -8.21
Kyte-Doolittle Hydrophobicity Value -0.211
Molecular Weight (kDa) 37.9
Isoelectric Point (pI) 5.72

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 439 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000318 (318 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0321

Transposon Insertions in PAO1

Type mini-Tn5 lux transposon insertion site (Non-transcriptional fusion)
Mutant ID UBC: PAO1_lux_50_C5
Genomic Position 360586
Position in ORF 174(1035)
Reference 15805499
Type mini-Tn5 lux transposon insertion site (Non-transcriptional fusion)
Mutant ID UBC: PAO1_lux_41_D3
Genomic Position 360597
Position in ORF 185(1035)
Reference 15805499
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 36636
Genomic Position 360538
Position in ORF 126(1035)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_04190
Mutant ID PA14NR: 47664
Genomic Position 375604
Position in ORF 1022(1035)
Reference 16477005

References

Crystallization and some properties of acetylpolyamine amidohydrolase from Mycoplana bullata.
Fujishiro K, Ando M, Uwajima T.
Biochem Biophys Res Commun. 1988 Dec 30;157(3):1169-74.
PubMed ID: 3207420
Acetylpolyamine amidohydrolase from Mycoplana ramosa: gene cloning and characterization of the metal-substituted enzyme.
Sakurada K, Ohta T, Fujishiro K, Hasegawa M, Aisaka K.
J Bacteriol. 1996 Oct;178(19):5781-6.
PubMed ID: 8824626
Transcriptome analysis of agmatine and putrescine catabolism in Pseudomonas aeruginosa PAO1.
Chou HT, Kwon DH, Hegazy M, Lu CD.
J Bacteriol. 2008 Mar;190(6):1966-75.
PubMed ID: 18192388
A fluorescence lifetime-based binding assay for acetylpolyamine amidohydrolases from Pseudomonas aeruginosa using a [1,3]dioxolo[4,5-f][1,3]benzodioxole (DBD) ligand probe.
Meyners C(1), Wawrzinek R, Krämer A, Hinz S, Wessig P, Meyer-Almes FJ.
Anal Bioanal Chem. 2014 Aug;406(20):4889-97.
PubMed ID: 24871864
Substrate specificity and function of acetylpolyamine amidohydrolases from Pseudomonas aeruginosa.
Krämer A(1), Herzer J(2), Overhage J(2), Meyer-Almes FJ(3).
BMC Biochem. 2016 Mar 9;17:4.
PubMed ID: 26956223